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Abstract Single-cell and single-nucleus RNA-sequencing technologies capture the expression of plant genes at an unprecedented resolution. Therefore, these technologies are gaining traction in plant molecular and developmental biology for elucidating the transcriptional changes across cell types in a specific tissue or organ, upon treatments, in response to biotic and abiotic stresses, or between genotypes. Despite the rapidly accelerating use of these technologies, collective and standardized experimental and analytical procedures to support the acquisition of high-quality data sets are still missing. In this commentary, we discuss common challenges associated with the use of single-cell transcriptomics in plants and propose general guidelines to improve reproducibility, quality, comparability, and interpretation and to make the data readily available to the community in this fast-developing field of research. 

ABSTRACT We present ALMA Band 7 polarization observations of the OMC-1 region of the Orion molecular cloud. We find that the polarization pattern observed in the region is likely to have been significantly altered by the radiation field of the >104 L⊙ high-mass protostar Orion Source I. In the protostar’s optically thick disc, polarization is likely to arise from dust self-scattering. In material to the south of Source I – previously identified as a region of ‘anomalous’ polarization emission – we observe a polarization geometry concentric around Source I. We demonstrate that Source I’s extreme luminosity may be sufficient to make the radiative precession time-scale shorter than the Larmor time-scale for moderately large grains ($$\gt 0.005\!-\!0.1\, \mu$$m), causing them to precess around the radiation anisotropy vector (k-RATs) rather than the magnetic field direction (B-RATs). This requires relatively unobscured emission from Source I, supporting the hypothesis that emission in this region arises from the cavity wall of the Source I outflow. This is one of the first times that evidence for k-RAT alignment has been found outside of a protostellar disc or AGB star envelope. Alternatively, the grains may remain aligned by B-RATs and trace gas infall on to the Main Ridge. Elsewhere, we largely find the magnetic field geometry to be radial around the BN/KL explosion centre, consistent with previous observations. However, in the Main Ridge, the magnetic field geometry appears to remain consistent with the larger-scale magnetic field, perhaps indicative of the ability of the dense Ridge to resist disruption by the BN/KL explosion. 

We present optical and near-infrared stellar polarization observations toward the dark filamentary clouds associated with IC5146. The data allow us to investigate the dust properties (this paper) and the magnetic field structure (Paper II). A total of 2022 background stars were detected in the R c , I\prime , H, and/or K bands to {A}V≲ 25 mag. The ratio of the polarization percentage at different wavelengths provides an estimate of {λ }\max , the wavelength of the peak polarization, which is an indicator of the small-size cutoff of the grain size distribution. The grain size distribution seems to significantly change at {A}V˜ 3 mag, where both the average and dispersion of {P}{Rc}/{P}H decrease. In addition, we found {λ }\max ˜ 0.6{--}0.9 μm for {A}V> 2.5 mag, which is larger than the ˜0.55 μm in the general interstellar medium (ISM), suggesting that grain growth has already started in low-A V regions. Our data also reveal that polarization efficiency ({PE}\equiv {P}λ /{A}V) decreases with A V as a power law in the R c , I\prime , and K bands with indices of -0.71 ± 0.10, -1.23 ± 0.10, and -0.53 ± 0.09. However, H-band data show a power index change; the PE varies with A V steeply (index of -0.95 ± 0.30) when {A}V< 2.88+/- 0.67 mag, but softly (index of -0.25 ± 0.06) for greater A V values. The soft decay of PE in high-A V regions is consistent with the radiative alignment torque model, suggesting that our data trace the magnetic field to {A}V˜ 20 mag. Furthermore, the breakpoint found in the H band is similar to that for A V , where we found the {P}{Rc}/{P}H dispersion significantly decreased. Therefore, the flat PE-A V in high-A V regions implies that the power-index changes result from additional grain growth. 

Abstract We have obtained sensitive dust continuum polarization observations at 850 μ m in the B213 region of Taurus using POL-2 on SCUBA-2 at the James Clerk Maxwell Telescope as part of the B -fields in STar-forming Region Observations (BISTRO) survey. These observations allow us to probe magnetic field ( B -field) at high spatial resolution (∼2000 au or ∼0.01 pc at 140 pc) in two protostellar cores (K04166 and K04169) and one prestellar core (Miz-8b) that lie within the B213 filament. Using the Davis–Chandrasekhar–Fermi method, we estimate the B -field strengths in K04166, K04169, and Miz-8b to be 38 ± 14, 44 ± 16, and 12 ± 5 μ G, respectively. These cores show distinct mean B -field orientations. The B -field in K04166 is well ordered and aligned parallel to the orientations of the core minor axis, outflows, core rotation axis, and large-scale uniform B -field, in accordance with magnetically regulated star formation via ambipolar diffusion taking place in K04166. The B -field in K04169 is found to be ordered but oriented nearly perpendicular to the core minor axis and large-scale B -field and not well correlated with other axes. In contrast, Miz-8b exhibits a disordered B -field that shows no preferred alignment with the core minor axis or large-scale field. We found that only one core, K04166, retains a memory of the large-scale uniform B -field. The other two cores, K04169 and Miz-8b, are decoupled from the large-scale field. Such a complex B -field configuration could be caused by gas inflow onto the filament, even in the presence of a substantial magnetic flux.