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Suspended finite ground coplanar waveguide (FG-CPW) interconnects, fabricated with laser-enhanced direct print additive manufacturing (AM), are modeled and characterized in this work. The study focuses on the variation of characteristic impedance and attenuation with design geometry. Acrylonitrile butadiene styrene (ABS) is printed with fused deposition modeling (FDM) to form 10-mm-long suspended ABS bridges and Dupont CB028 is microdispensed to realize conductive traces on the ABS bridges. Femtosecond pulsed laser machining in the ultraviolet range is combined with the AM to create gaps ranging from 8 to 92 μ m in width on either side of a signal line to define the FG-CPW. Three different suspended interconnects are designed, where the total linewidth (signal line plus gaps) is kept constant at 300 μ m for all designs, but the aspect ratio (AR) (signal linewidth divided by total linewidth) is varied. Two multiline thru–reflect–line calibrations are performed to measure each design: one uses printed calibration standards and the other employs a commercial calibration substrate. The attenuation of the interconnects at 30 GHz is 0.28, 0.13, and 0.06 dB/mm for ARs of 0.95, 0.87, and 0.38, respectively. The laser machining of the gaps results in partial substrate removal, which increases the characteristic impedance by approximately 11%. The impact of fabrication tolerances is examined. 

Triple-negative breast cancer (TNBC) is a highly metastatic subtype of breast cancer. The epithelial-to-mesenchymal transition is a nonbinary process in the metastatic cascade that generates tumor cells with both epithelial and mesenchymal traits known as hybrid EM cells. Recent studies have elucidated the enhanced metastatic potential of cancers featuring the hybrid EM phenotype, highlighting the need to uncover molecular drivers and targetable vulnerabilities of the hybrid EM state. Here, we discovered that hybrid EM breast tumors are enriched in CD38, an immunosuppressive molecule associated with worse clinical outcomes in liquid malignancies. Altering CD38 expression in tumor cell impacted migratory, invasive, and metastatic capabilities of hybrid EM cells. Abrogation of CD38 expression stimulated an antitumor immune response, thereby preventing the generation of an immunosuppressive microenvironment in hybrid EM tumors. CD38 levels positively correlated with PD-L1 expression in samples from patients with TNBC. Moreover, targeting CD38 potentiated the activity of anti–PD-L1, eliciting strong antitumor immunity, with reduced tumor growth in hybrid EM models. Overall, this research exposes upregulation of CD38 as a specific survival strategy utilized by hybrid EM breast tumors to suppress immune cell activity and sustain metastasis, with strong implications in other carcinomas that have hybrid EM properties.

Significance: Hybrid cells co-featuring epithelial and mesenchymal traits in triple-negative breast cancer express elevated levels of CD38 to induce immunosuppression and metastasis, indicating CD38 inhibition as potential strategy for treating breast cancer.

 

Laser enhanced direct print additive manufacturing (LE-DPAM) technology has recently been demonstrated to achieve success in packaging of antennas with phase shifters to realize passive phased antenna arrays (PAAs). Utilizing LE-DPAM for PAAs operating in mm-wave bands brings out new challenges that need to be addressed. These challenges are associated with smaller antenna and feature sizes needed for mm-wave band operation, necessity of active circuits for amplification, and number of pads, pad size and pad locations of mm-wave beamformer IC packages. This paper presents our initial progress in scaling LE-DPAM based packaging of PAAs into the mm-wave band operation through consideration and demonstration of discrete components (i.e. antenna array elements and beamformer ICs) that form the PAA structure. Specifically, a stand-alone, passive, 2×2 LE-DPAM based 26 GHz antenna subarray is investigated for its performance. In addition, a 24.5 GHz – 27 GHz beamformer IC is packaged in a stand-alone test article using LE-DPAM and investigated for its mm-wave performance and thermal aspects. 

Marine biofouling is a complex and dynamic process that significantly increases the carbon emissions from the maritime industry by increasing drag losses. However, there are no existing non‐toxic marine paints that can achieve both effective fouling reduction and efficient fouling release. Inspired by antifouling strategies in nature, herein, a superoleophobic zwitterionic nanowire coating with a nanostructured hydration layer is introduced, which exhibits simultaneous fouling reduction and release performance. The zwitterionic nanowires demonstrate >25% improvement in fouling reduction compared to state‐of‐the‐art antifouling nanostructures, and four times higher fouling‐release compared to conventional zwitterionic coatings. Fouling release is successfully achieved under a wall shear force that is four orders of magnitude lower than regular water jet cleaning. The mechanism of this simultaneous fouling reduction and release behavior is explored, and it is found that a combination of 1) a mechanical biocidal effect from the nanowire geometry, and 2) low interfacial adhesion resulting from the nanostructured hydration layer, are the major contributing factors. These findings provide insights into the design of nanostructured coatings with simultaneous fouling reduction and release. The newly established synthesis procedure for the zwitterionic nanowires opens new pathways for implementation as antifouling coatings in the maritime industry and biomedical devices. 

Morphological novelties, or key innovations, are instrumental to the diversification of the organisms. In plants, one such innovation is the evolution of zygomorphic flowers, which is thought to promote outcrossing and increase flower morphological diversity. We isolated three allelic mutants from twoMimulusspecies displaying altered floral symmetry and identified the causal gene as the ortholog ofArabidopsis BLADE-ON-PETIOLE. We found that MlBOP and MlCYC2A physically interact and this BOP-CYC interaction module is highly conserved across the angiosperms. Furthermore, MlBOP self-ubiquitinates and suppressesMlCYC2Aself-activation. MlCYC2A, in turn, impedes MlBOP ubiquitination. Thus, this molecular tug-of-war between MlBOP and MlCYC2A fine-tunes the expression ofMlCYC2A, contributing to the formation of bilateral symmetry in flowers, a key trait in angiosperm evolution.

 

Drought stress poses a substantial challenge to plant growth and agricultural productivity worldwide. Upon water depletion, plants activate an abscisic acid (ABA) signaling pathway, leading to stomatal closure to reduce water loss. The MYB family of transcription factors plays diverse roles in growth, development, stress responses, and biosynthesis, yet their involvement in stomatal regulation remains unclear. Here, we demonstrate that ABA significantly upregulates the expression of MYB41, MYB74, and MYB102, with MYB41 serving as a key regulator that induces the expression of both MYB74 and MYB102. Through luciferase assays, chromatin immunoprecipitation (ChIP) assays, and electrophoretic mobility shift assays (EMSA), we reveal that MYB41 engages in positive feedback regulation by binding to its own promoter, thus amplifying its transcription in Arabidopsis (Arabidopsis thaliana). Furthermore, our investigation showed that MYB41 recruits BRAHMA (BRM), the core ATPase subunit of the SWI/SNF complex, to the MYB41 promoter, facilitating the binding of HISTONE DEACETYLASE 6 (HDA6). This recruitment triggers epigenetic modifications, resulting in reduced MYB41 expression characterized by elevated H3K27me3 levels and concurrent decreases in H3ac, H3K27ac, and H3K14ac levels in wild-type plants compared to brm knockout mutant plants. Our genetic and molecular analyses show that ABA mediates autoregulation of the MYB41-BRM module, which intricately modulates stomatal movement in A. thaliana. This discovery sheds light on a drought response mechanism with the potential to greatly enhance agricultural productivity.