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Abstract Despite major advances in HIV testing, ultrasensitive detection of early infection remains challenging, especially for the viral capsid protein p24, which is an early virological biomarker of HIV-1 infection. Here, To improve p24 detection in patients missed by immunological tests that dominate the diagnostics market, we show a click chemistry amplified nanopore (CAN) assay for ultrasensitive quantitative detection. This strategy achieves a 20.8 fM (0.5 pg/ml) limit of detection for HIV-1 p24 antigen in human serum, demonstrating 20~100-fold higher analytical sensitivity than nanocluster-based immunoassays and clinically used enzyme-linked immunosorbent assay, respectively. Clinical validation of the CAN assay in a pilot cohort shows p24 quantification at ultra-low concentration range and correlation with CD4 count and viral load. We believe that this strategy can improve the utility of p24 antigen in detecting early infection and monitoring HIV progression and treatment efficacy, and also can be readily modified to detect other infectious diseases. 

Multimode fibers are explored widely for optical communication, spectroscopy, imaging, and sensing applications. Here we demonstrate a single-shot full-field temporal measurement technique based on a multimode fiber. The complex spatiotemporal speckle field is created by a reference pulse propagating through the fiber, and it interferes with a signal pulse. From the time-integrated interference pattern, both the amplitude and the phase of the signal are retrieved. The simplicity and high sensitivity of our scheme illustrate the potential of multimode fibers as versatile and multi-functional sensors. 

Characterizing ultrashort optical pulses has always been a critical but difficult task, which has a broad range of applications. We propose and demonstrate a self-referenced method of characterizing ultrafast pulses with a multimode fiber. The linear and nonlinear speckle patterns formed at the distal end of a multimode fiber are used to recover the spectral amplitude and phase of an unknown pulse. We deploy a deep learning algorithm for phase recovery. The diversity of spatial and spectral modes in a multimode fiber removes any ambiguity in the sign of the recovered spectral phase. Our technique allows for single-shot pulse characterization in a simple experimental setup. This work reveals the potential of multimode fibers as a versatile and multi-functional platform for optical sensing. 

Abstract Long-range correlations play an essential role in wave transport through disordered media, but have rarely been studied in other complex systems. Here we discover spatio-temporal intensity correlations for an optical pulse propagating through a multimode fiber with strong random mode coupling. Positive long-range correlation arises from multiple scattering in fiber mode space and depends on the statistical distribution of arrival times. By optimizing the incident wavefront of a pulse, we maximize the power transmitted at a selected time, and such control is significantly enhanced by the long-range spatio-temporal correlation. We provide an explicit relation between the correlation and the power enhancement, which agrees with experimental results. Our work shows that multimode fibers provide a fertile ground for studying complex wave phenomena. The strong spatio-temporal correlation can be employed for efficient power delivery at a well-defined time.