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The study of ancient DNA is revolutionizing our understanding of paleo-ecology and the evolutionary history of species. Insects are essential components in many ecosystems and constitute the most diverse group of animals. Yet they are largely neglected in ancient DNA studies. We report the results of the first targeted investigation of insect ancient DNA to positively identify subfossil insects to species, which includes the recovery of endogenous content from samples as old as ~ 34,355 ybp. Potential inhibitors currently limiting widespread research on insect ancient DNA are discussed, including the lack of closely related genomic reference sequences (decreased mapping efficiency) and the need for more extensive collaborations with insect taxonomists. The advantages of insect-based studies are also highlighted, especially in the context of understanding past climate change. In this regard, insect remains from ancient packrat middens are a rich and largely uninvestigated resource for exploring paleo-ecology and species dynamics over time.

 

Abstract Sepidiini is a speciose tribe of desert-inhabiting darkling beetles, which contains a number of poorly defined taxonomic groups and is in need of revision at all taxonomic levels. In this study, two previously unrecognized lineages were discovered, based on morphological traits, among the extremely speciose genera Psammodes Kirby, 1819 (164 species and subspecies) and Ocnodes Fåhraeus, 1870 (144 species and subspecies), namely the Psammodes spinosus species-group and Ocnodes humeralis species-group. In order to test their phylogenetic placement, a phylogeny of the tribe was reconstructed based on analyses of DNA sequences from six nonoverlapping genetic loci (CAD, wg, COI JP, COI BC, COII, and 28S) using Bayesian and maximum likelihood inference methods. The aforementioned, morphologically defined, species-groups were recovered as distinct and well-supported lineages within Molurina + Phanerotomeina and are interpreted as independent genera, respectively, Tibiocnodes Gearner & Kamiński gen. nov. and Tuberocnodes Gearner & Kamiński gen. nov. A new species, Tuberocnodes synhimboides Gearner & Kamiński sp. nov., is also described. Furthermore, as the recovered phylogenetic placement of Tibiocnodes and Tuberocnodes undermines the monophyly of Molurina and Phanerotomeina, an analysis of the available diagnostic characters for those subtribes is also performed. As a consequence, Phanerotomeina is considered as a synonym of the newly redefined Molurina sens. nov. Finally, spectrograms of vibrations produced by substrate tapping of two Molurina species, Toktokkus vialis (Burchell, 1822) and T. synhimboides, are presented. 

A new species of the genus Ocnodes is described (O. kruegeri spec. nov.) from Namaqualand, southern Africa. Moreover, an update of the recently published catalogue (Kaminski et al., 2019a) of the tribe Sepidiini is provided. This includes discovery of two nomenclatural duplications and inclusion of nine previously omitted species. The following new combinations are proposed (both species transferred from Psammodes): Ocnodes badeni (Haag-Rutenberg, 1871) and Ocnodes damarinus (Péringuey, 1904). Trachynotus kruegeri nom. nov. is introduced for Trachynotus vicinus Péringuey, 1886 to avoid homonymy with Trachynotus vicinus (Haag-Rutenberg, 1871). 

Abstract The first molecular phylogeny of the tribe Sepidiini is inferred from analyses of DNA sequence data from the following five loci (CAD, wg, COI, COII, 28S rRNA). Bayesian and maximum likelihood analyses were performed on a dataset containing 41 taxa, of which a majority represent Molurina (27) and Phanerotomeina (6). The resulting topologies were used to discuss phylogenetic placement and diagnostic characters of all of the genera representing Molurina. Within the subtribe, the results revealed paraphyly of the genus Psammodes. The P. vialis species-group, currently classified within Psammodes, was recovered as sister to all other Molurina genera. Based on this topology and morphological investigations, a new genus named Toktokkus gen. nov. is established. Within Phanerotomeina, Ocnodes is paraphyletic with regard to Tarsocnodes. In order to restore the monophyly of Ocnodes, the subgenus Chiliarchum stat. nov. is elevated to generic level. Finally, as the homology of female terminalia structures has never been fully assessed for Sepidiini, a comparative study of ovipositor morphology was conducted. As a result, this paper presents the first fully annotated ovipositors for tok-tokkie beetles. 

The darkling beetle genus Hypogena Dejean, 1834 (Tenebrionidae: Tenebrioninae) is revised. Hypogena is entirely composed of dorsoventrally flattened species that live subcortically in dead trees. This genus is generally identified by male specific characters, particularly the presence of cephalic horns. Hypogena is currently placed within the tribe Triboliini Gistel, 1848. However, several previously overlooked morphological characters call into question its placement within the tribe. A morphological matrix of 94 external adult characters was assembled to examine species relationships and boundaries. The resulting phylogeny is presented. Thirteen Hypogena species were previously recognized as valid, including Hypogena marginalis Doyen & Poinar from Dominican amber. Four previously unidentified species are described in this study: Hypogena akuma sp. nov. (Brazil), Hypogena cryptica sp. nov. (Mexico), Hypogena hirsuta sp. nov. (Ecuador), and Hypogena reburra sp. nov. (Colombia). Lectotypes are designated for Hypogena depressa (Champion, 1886), Hypogena dejeani (Champion, 1886), Hypogena canaliculata (Champion, 1886), and Hypogena vacca (Fabricius, 1801). A neotype is designated for Tenebrio biimpressus (Latreille, 1833) (type species of Hypogena, synonymized under Hypogena brasilica (Perty)) in order to maintain stability within the genus. 

A new species of Stenosini (Coleoptera: Tenebrionidae: Pimeliinae), Schizaraeus fouquei sp. nov. is described from southern Andes of Peru: Apurímac and Cusco regions. Prior to this study, Schizaraeus Kulzer, 1955 was monotypic with one species, S. acuticosta Kulzer, 1955 from Catamarca Province in Argentina. We present habitus photographs of both species, scanning electron micrographs of external morphology of the new species, a distribution map, and a comparison of the characters of both species of the genus. 

The taxonomic concept of the genus Machleida Fåhraeus, 1870 is tested and revised based on newly identified material. The following new species are described: Machleida banachi , M. flagstaffensis , M. tarskii , and M. zofiae Kamiński. Machleida capillosa Wilke, 1925 is considered as a junior subjective synonym of Asida devia Péringuey, 1899. Asida lecta Péringuey, 1899 (= Pseudomachla recurva Wilke, 1925) (transferred to Afrasida ), Machleida nossibiana Fairmaire, 1897 (transferred to Scotinesthes ), and Machleida tuberosa Wilke, 1925 (interpreted as incertae sedis in Asidini) are excluded from Machleida . An identification key for the species of the newly revised Machleida is provided. The present paper brings the total number of species within the genus to six ( M. banachi sp. nov. ; M. devia (Péringuey, 1899); M. flagstaffensis sp. nov. ; M. nodulosa Fåhraeus, 1870; M. tarskii sp. nov. ; M. zofiae Kamiński sp. nov. ). The morphology of female terminalia (ovipositor and genital tubes) is described for the genus for the first time.