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Abstract Quantifying nitrate leaching in agricultural fields is often complicated by inability to capture all water draining through a specific area. We designed and tested undisturbed soil monoliths (termed “soil block mesocosms”) to achieve complete collection of drainage. Each mesocosm measures 1.5 m × 1.5 m × 1.2 m and is enclosed by steel on the sides and bottom with a single outlet to collect drainage. We compared measurements from replicate mesocosms planted to corn (Zea maysL.) with a nearby field experiment with tile‐drained plots (“drainage plots”), and with drainage from nearby watersheds from 2020 through 2022 under drought conditions. Annual mesocosm drainage volumes were 6.5–24.6 cm greater than from the drainage plots, likely because the mesocosms were isolated from the subsoil and could not store groundwater below the drain depth, whereas the drainage plots accumulated infiltration as groundwater. Thus, we obtained consistent nitrate leaching measurements from the mesocosms even when some drainage plots yielded no water. Despite drainage volume differences, mean flow‐weighted nitrate concentrations were similar between mesocosms and drainage plots in 2 of 3 years. Mesocosm annual drainage volume was 8.7 cm lower to 16.7 cm higher than watershed drainage, likely due to lagged influences of groundwater. Corn yields were lower in mesocosms than drainage plots in 2020, but with irrigation, yields were similar in subsequent years. Mean 2020 surface soil moisture and temperature were similar between the mesocosms and nearby fields. Based on these comparisons, the mesocosms provide a robust method to measure nitrate leaching with lower variability than field plots.more » « less
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Abstract This study compares a real choice experiment (RCE) with three commonly used experimental auction (EA) mechanisms (Becker–DeGroot–Marschak, random nth price auction (RNPA), second price auction (SPA)) to determine whether willingness to pay (WTP) estimates differ across these elicitation methods. We use quality labels on eggs as the empirical application and find that the SPA, RNPA and RCE yield similar WTP estimates, while the BDM mechanism generally produces higher WTP estimates. We also compare these EAs and the RCE in an induced value setting and find that the BDM auction produces greater deviations from the underlying value than the other EAs and RCE. We suggest that RCEs may be preferable to BDMs for collecting WTP estimates in logistically difficult experimental settings.more » « less
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Abstract Fresh pork tenderness contributes to consumer satisfaction with the eating experience. Postmortem proteolysis of proteins within and between myofibrils has been closely linked with pork tenderness development. A clear understanding of the molecular features associated with pork tenderness development will provide additional targets and open the door to new solutions to improve and make pork tenderness development more consistent. Therefore, the objective was to utilize liquid chromatography and mass spectrometry with tandem mass tag (TMT) multiplexing to evaluate myofibrillar sub-proteome differences between pork chops of different instrumental star probe values. Pork loins (N = 120) were collected from a commercial harvest facility at 24 h postmortem. Quality and sensory attributes were evaluated at 24 h postmortem and after ~2 weeks of postmortem aging. Pork chops were grouped into 4 groups based on instrumental star probe value (group A,x¯ = 4.23 kg, 3.43 to 4.55 kg; group B,x¯ = 4.79 kg, 4.66 to 5.00 kg; group C,x¯ = 5.43 kg, 5.20 to 5.64 kg; group D,x¯ = 6.21 kg, 5.70 to 7.41 kg; n = 25 per group). Myofibrillar proteins from the samples aged ~2 wk were fractionated, washed, and solubilized in 8.3 M urea, 2 M thiourea, and 1% dithiothreitol. Proteins were digested with trypsin, labeled with 11-plex isobaric TMT reagents, and identified and quantified using a Q-Exactive Mass Spectrometer. Between groups A and D, 54 protein groups were differentially abundant (adjusted P < 0.05). Group A had a greater abundance of proteins related to the thick and thin filament and a lesser abundance of Z-line-associated proteins and metabolic enzymes than group D chops. These data highlight that distinct myofibrillar sub-proteomes are associated with pork chops of different tenderness values. Future research should evaluate changes immediately and earlier postmortem to further elucidate myofibrillar sub-proteome differences over the postmortem aging period.more » « less
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Abstract As the levels of plastic use in global society have increased, it has become crucial to regulate plastics of all sizes including both microplastics (MPs) and nanoplastics (NPs). Here, the published literature on the current laws passed by the US Congress and regulations developed by various federal agencies such as the US Environmental Protection Agency and the US Food and Drug Administration (FDA) that could be used to regulate MPs and NPs have been reviewed and analyzed. Statutes such as the Clean Water Act, the Safe Drinking Water Act, the Toxic Substances Control Act (TSCA), the Resource Conservation and Recovery Act, and the Clean Air Act can all be used to address plastic pollution. These statutes have not been invoked for MP and NP waste in water or air. The Federal Food, Drug, and Cosmetic Act provides guidance on how the FDA should evaluate plastics use in food, food packaging, cosmetics, drug packaging, and medical devices. The FDA has recommended that acceptable levels of ingestible contaminant from recycled plastic are less than 1.5 µg/person/day, which is 476 000 times less than the possible ingested daily dose. Plastic regulation is present at the state level. States have banned plastic bags, and several cities have banned plastic straws. California is the only state beginning to focus on monitoring MPs in drinking water. The future of MP regulation in the USA should use TSCA to test the safety of plastics. The other statutes need to include MPs in their definitions. For the FDA, MPs should be redefined as contaminants—allowing tolerances to be set for MPs in food and beverages. Through minor changes in how MPs are classified, it is possible to begin to use the current statutes to understand and begin to minimize the possible effects of MPs on human health and the environment. Integr Environ Assess Manag 2023;19:474–488. © 2022 The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).more » « less
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Free, publicly-accessible full text available December 1, 2025
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There is limited peer-reviewed research exploring trust in nutritional science. Given the importance of trust in health scientists on the amount of attention paid to guidelines and participation in healthy behaviors, it’s important to understand how the public trusts our field. We address this gap by studying how trust in nutritional science is moderated by demographics such as political and religious beliefs and by comparing trust in nutritional science to other scientific fields that study our food system.more » « less
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This study presents a county-level analysis and visual assessment of Iowa’s Food–Energy–Water systems (IFEWs), focusing on the interdependencies of agricultural practices, nitrogen management, and energy production. We use data from the USDA National Agricultural Statistics Service and other sources to assess cropland-nitrogen sources, animal-based-nitrogen contributions, and ethanol production capacity across Iowa’s counties. Our methodology leverages geoprocessing and interpolation tools to address data availability challenges and refine nitrogen surplus (Ns) estimates. The results reveal spatial–temporal dynamics of cropland-nitrogen, quantify non-point nitrogen sources at the county level, and evaluate the impact of energy systems on the IFEWs balance. We discuss the implications of our findings for sustainable agriculture, environmental management, and energy production in Iowa. The study highlights the need for integrated approaches to address the complex interactions within the IFEWs and informs policy development for sustainable resource management.more » « less
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