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ABSTRACT Fungal contamination of drinking water distribution systems can impact water quality with implications for public health. We document an instance of Exophiala spp. biofilm contamination of customer taps in the Midwest United States following consumer complaints. Three samples of black biofilm were collected from customer taps in Ohio and then processed using next-generation DNA sequencing of the bacterial 16S and fungal ITS regions. Two samples with successful ITS sequencing were dominated by Exophiala spp., putatively identified as E. cancerae and E. lecanii-corni. Dominant bacterial phyla in samples included Proteobacteria, Bacteroidetes, Actinobacteria, and Acidobacteria. Bacterial composition varied substantially at the family and genus levels, and potentially pathogenic bacteria (i.e., Acinetobacter spp., Legionella spp., Mycobacterium spp., and Pseudomonas spp.) were detected. The potential for fungal contamination of drinking water distribution systems should be evaluated when biofilms are observed.