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  1. In the introduced range, the successful establishment of an invasive species may be influenced by positive plant-plant interactions. Pre-existing vegetation, particularly conspecific mature trees, can shape the availability and attributes of microsites, thus potentially creating favorable conditions for the establishment of conspecific seedlings through facilitation. Pines are widely introduced in the Southern Hemisphere primarily as forestry plantations; these pines can become invasive, causing detrimental effects on local ecosystems and economies. In the high-elevation grasslands of the Sierras de Córdoba, Central Argentina, pines have begun to invade the native grassland as a result of improper (or lack of) management of pine plantations. During early pine invasion in this semi-arid grassland, we aimed to quantify the influence of adult live pines and on congeneric pine seedling recruitment and survival. For this, 48,000 seeds of Pinus elliottii and P. taeda were sown in three consecutive field trials, under different tree status treatments: live pines, dead pines, and no pines (i.e., open grassland). Seed were sown with and without irrigation and seeded microsites were oriented to the north and south of the live and dead trees. We also considered the hillslope aspect where the sites were located. Our results show that pine seedling recruitment was 57 % higher under live pines compared to dead pines and no pines treatment, but only in the trials that were not irrigated. Microsites south of the live pine trees, more shaded from direct sunlight in the Southern hemisphere, presented 36 % more pine seed germination than those to the north. In terms of topography, hillslope aspects with lower solar incidence (wetter hillslopes) also showed higher pine seedling recruitment. Our results suggest that moisture availability is a dominant factor driving further invasion, and that adult pines may be facilitating the invasion process by creating moister microsites for germination and pine seedling establishment. Thus, the early removal of adult pines is important to consider in the management of pine invasions. They do not only serve as a source of propagules, but also positively affect the establishment of their congeneric seedlings. 
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  2. null (Ed.)