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Recovering nitrogen (N) from municipal wastewater is a promising approach to prevent nutrient pollution, reduce energy use, and transition toward a circular N bioeconomy, but remains a technologically challenging endeavor. Existing N recovery techniques are optimized for high-strength, low-volume wastewater. Therefore, developing methods to concentrate dilute N from mainstream wastewater will bridge the gap between existing technologies and practical implementation. The N-rich biopolymer cyanophycin is a promising candidate for N bioconcentration due to its pH-tunable solubility characteristics and potential for high levels of accumulation. However, the cyanophycin synthesis pathway is poorly explored in engineered microbiomes. In this study, we analyzed over 3,700 publicly available metagenome assembled genomes (MAGs) and found that the cyanophycin synthesis genecphAwas ubiquitous across common activated sludge bacteria. We found thatcphAwas present in common phosphorus accumulating organisms (PAO)Ca.‘Accumulibacter’ andTetrasphaera,suggesting potential for simultaneous N and P bioconcentration in the same organisms. Using metatranscriptomic data, we confirmed the expression ofcphAin lab-scale bioreactors enriched with PAO. Our findings suggest that cyanophycin synthesis is a ubiquitous metabolic activity in activated sludge microbiomes. The possibility of combined N and P bioconcentration could lower barriers to entry for N recovery, since P concentration by PAO is already a widespread biotechnology in municipal wastewater treatment. We anticipate this work to be a starting point for future evaluations of combined N and P bioaccumulation, with the ultimate goal of advancing widespread adoption of N recovery from municipal wastewater. 

Increasingly, circularity indicators for material, energy, and water systems guide circular economy design. While indicators for products made from recycled carbon-based materials are somewhat common, peer indicators for waste nitrogen-derived products are limited. It is important, however, to develop such indicators to guide emerging technologies that transform waste nitrogen into products. In this study, we summarize the nitrogen circularity indicator literature, emphasizing the agricultural and wastewater sectors. Next, we use the Material Circularity Indicator (MCI) developed by the Ellen MacArthur Foundation, to quantify the circularity of products made from waste nitrogen in swine manure. We considered four test cases using different technologies to recover nitrogen from the manure. Our analysis indicates that technologies that seem to increase circularity on the surface may not yield a substantial increase in MCI results. Finally, we discuss the strengths and weaknesses of using the MCI for product-level analysis and further developments. 

The nitrogen cycle needed for scaled agriculture relies on energy- and carbon-intensive processes and generates nitrate-containing wastewater. Here we focus on an alternative approach—the electrified co-electrolysis of nitrate and CO2 to synthesize urea. When this is applied to industrial wastewater or agricultural runoff, the approach has the potential to enable low-carbon-intensity urea production while simultaneously providing wastewater denitrification. We report a strategy that increases selectivity to urea using a hybrid catalyst: two classes of site independently stabilize the key intermediates needed in urea formation, *CO2NO2 and *COOHNH2, via a relay catalysis mechanism. A Faradaic efficiency of 75% at wastewater-level nitrate concentrations (1,000 ppm NO3− [N]) is achieved on Zn/Cu catalysts. The resultant catalysts show a urea production rate of 16 µmol h−1 cm−2. Life-cycle assessment indicates greenhouse gas emissions of 0.28 kg CO2e per kg urea for the electrochemical route, compared to 1.8 kg CO2e kg−1 for the present-day route.