- NSF-PAR ID:
- 10030913
- Publisher / Repository:
- DOI PREFIX: 10.1029
- Date Published:
- Journal Name:
- Journal of Geophysical Research: Oceans
- Volume:
- 122
- Issue:
- 7
- ISSN:
- 2169-9275
- Page Range / eLocation ID:
- p. 5850-5869
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
More Like this
-
Abstract We present differential Strömgren
uvby observations from the Four College Automated Photometric Telescope (FCAPT) in Washington Camp, AZ of eight magnetic Chemically Peculiar (mCP) stars: HD 32966, HD 35298, HD 68292, HD 93226, HD 171247, HD 217833, HD 220147, and HD 223358. We use multiple period‐finding algorithms and incorporate data from the ESA Hipparcos catalogue to study the amplitudes, periods, and asymmetries in the light curves. No previous FCAPT data have been published for HD 68292. For the seven other stars, these studies substantially extend the analyses of published FCAPT datasets. -
Abstract Tropical forests are well known for their high woody plant diversity. Processes occurring at early life stages are thought to play a critical role in maintaining this high diversity and shaping the composition of tropical tree communities. To evaluate hypothesized mechanisms promoting tropical tree species coexistence and influencing composition, we initiated a census of woody seedlings and small saplings in the permanent 50 ha Forest Dynamics Plot (FDP) on Barro Colorado Island (BCI), Panama. Situated in old‐growth, lowland tropical moist forest, the BCI FDP was originally established in 1980 to monitor trees and shrubs ≥1 cm diameter at 1.3 m above ground (dbh) at ca. 5‐year intervals. However, critical data on the dynamics occurring at earlier life stages were initially lacking. Therefore, in 2001 we established a 1‐m2seedling plot in the center of every 5 × 5 m section of the BCI FDP. All freestanding woody individuals ≥20 cm tall and <1 cm dbh (hereafter referred to as seedlings) were tagged, mapped, measured, and identified to species in 19,313 1‐m2seedling plots. Because seedling dynamics are rapid, we censused these seedling plots every 1–2 years. Here, we present data from the 14 censuses of these seedling plots conducted between the initial census in 2001 to the most recent census, in 2018. This data set includes nearly 1 M observations of ~185,000 individuals of >400 tree, shrub, and liana species. These data will permit spatially‐explicit analyses of seedling distributions, recruitment, growth, and survival for hundreds of woody plant species. In addition, the data presented here can be linked to openly‐available, long‐term data on the dynamics of trees and shrubs ≥1 cm dbh in the BCI FDP, as well as existing data sets from the site on climate, canopy structure, phylogenetic relatedness, functional traits, soil nutrients, and topography. This data set can be freely used for non‐commercial purposes; we request that users of these data cite this data paper in all publications resulting from the use of this data set.
-
Summary The mitochondrial and chloroplast
mRNA s of the majority of land plants are modified through cytidine to uridine (C‐to‐U)RNA editing. Previously, forward and reverse genetic screens demonstrated a requirement for pentatricopeptide repeat (PPR ) proteins forRNA editing. Moreover, chloroplast editing factorsOZ 1,RIP 2,RIP 9 andORRM 1 were identified in co‐immunoprecipitation (co‐IP) experiments, albeit the minimal complex sufficient for editing activity was never deduced. The current study focuses on isolated, intact complexes that are capable of editing distinct sites. Peak editing activity for four sites was discovered in size‐exclusion chromatography (SEC) fractions ≥ 670 kDa, while fractions estimated to be approximately 413 kDa exhibited the greatest ability to convert a substrate containing the editing siterps14 C80.RNA content peaked in the ≥ 670 kDa fraction. Treatment of active chloroplast extracts withRN ase A abolished the relationship of editing activity with high‐MW fractions, suggesting a structuralRNA component in native complexes. By immunoblotting,RIP 9,OTP 86,OZ 1 andORRM 1 were shown to be present in active gel filtration fractions, thoughOZ 1 andORRM 1 were mainly found in low‐MW inactive fractions. Active editing factor complexes were affinity‐purified using anti‐RIP 9 antibodies, and orthologs to putativeArabidopsis thaliana RNA editing factorPPR proteins,RIP 2,RIP 9,RIP 1,OZ 1,ORRM 1 andISE 2 were identified via mass spectrometry. Western blots from co‐IP studies revealed the mutual association ofOTP 86 andOZ 1 with nativeRIP 9 complexes. Thus,RIP 9 complexes were discovered to be highly associated with C‐to‐URNA editing activity and other editing factors indicative of their critical role in vascular plant editosomes.