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1. Growing Degree-Day Measurement of Cyst Germination Rates in the Toxic Dinoflagellate Alexandrium catenella

   https://doi.org/10.1128/aem.02518-21  

   Fischer, Alexis D. ; Brosnahan, Michael L. ( June 2022 , Applied and Environmental Microbiology)

   Rudi, Knut (Ed.)

   ABSTRACT Blooms of many dinoflagellates, including several harmful algal bloom (HAB) species, are seeded and revived through the germination of benthic resting cysts. Temperature is a key determinant of cysts’ germination rate, and temperature–germination rate relationships are therefore fundamental to understanding species’ germling cell production, cyst bed persistence, and resilience to climate warming. This study measured germination by cysts of the HAB dinoflagellate Alexandrium catenella using a growing degree-day ( DD ) approach that accounts for the time and intensity of warming above a critical temperature. Time courses of germination at different temperatures were fit to lognormal cumulative distribution functions for the estimation of the median days to germination. As temperature increased, germination times decreased hyperbolically. DD scaling collapsed variability in germination times between temperatures after cysts were oxygenated. A parallel experiment demonstrated stable temperature–rate relationships in cysts collected during different phases of seasonal temperature cycles in situ over three years. DD scaling of the results from prior A. catenella germination studies showed consistent differences between populations across a wide range of temperatures and suggests selective pressure for different germination rates. The DD model provides an elegant approach to quantify and compare the temperature dependency of germination among populations, between species, and in response to changing environmental conditions. IMPORTANCE Germination by benthic life history stages is the first step of bloom initiation in many, diverse phytoplankton species. This study outlines a growing degree-day ( DD ) approach for comparing the temperature dependence of germination rates measured in different populations. Germination by cysts of Alexandrium catenella , a harmful algal bloom dinoflagellate that causes paralytic shellfish poisoning, is shown to require a defined amount of warming, measured in DD after cysts are aerated. Scaling by DD , the time integral of temperature difference from a critical threshold, enabled direct comparison of rates measured at different temperatures and in different studies. 

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2. Evidence for massive and recurrent toxic blooms of Alexandrium catenella in the Alaskan Arctic

   https://doi.org/10.1073/pnas.2107387118  

   Anderson, Donald M. ; Fachon, Evangeline ; Pickart, Robert S. ; Lin, Peigen ; Fischer, Alexis D. ; Richlen, Mindy L. ; Uva, Victoria ; Brosnahan, Michael L. ; McRaven, Leah ; Bahr, Frank ; et al ( October 2021 , Proceedings of the National Academy of Sciences)

   Among the organisms that spread into and flourish in Arctic waters with rising temperatures and sea ice loss are toxic algae, a group of harmful algal bloom species that produce potent biotoxins. Alexandrium catenella , a cyst-forming dinoflagellate that causes paralytic shellfish poisoning worldwide, has been a significant threat to human health in southeastern Alaska for centuries. It is known to be transported into Arctic regions in waters transiting northward through the Bering Strait, yet there is little recognition of this organism as a human health concern north of the Strait. Here, we describe an exceptionally large A. catenella benthic cyst bed and hydrographic conditions across the Chukchi Sea that support germination and development of recurrent, locally originating and self-seeding blooms. Two prominent cyst accumulation zones result from deposition promoted by weak circulation. Cyst concentrations are among the highest reported globally for this species, and the cyst bed is at least 6× larger in area than any other. These extraordinary accumulations are attributed to repeated inputs from advected southern blooms and to localized cyst formation and deposition. Over the past two decades, warming has likely increased the magnitude of the germination flux twofold and advanced the timing of cell inoculation into the euphotic zone by 20 d. Conditions are also now favorable for bloom development in surface waters. The region is poised to support annually recurrent A. catenella blooms that are massive in scale, posing a significant and worrisome threat to public and ecosystem health in Alaskan Arctic communities where economies are subsistence based. 

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3. Evaluation of sxtA and rDNA qPCR assays through monitoring of an inshore bloom of Alexandrium catenella Group 1

   https://doi.org/10.1038/s41598-019-51074-3  

   Murray, Shauna A. ; Ruvindy, Rendy ; Kohli, Gurjeet S. ; Anderson, Donald M. ; Brosnahan, Michael L. ( December 2019 , Scientific Reports)
4. Spatiotemporal genetic structure of regional-scale Alexandrium catenella dinoflagellate blooms explained by extensive dispersal and environmental selection

   https://doi.org/10.1016/j.hal.2019.03.013  

   Gao, Yida ; Sassenhagen, Ingrid ; Richlen, Mindy L. ; Anderson, Donald M. ; Martin, Jennifer L. ; Erdner, Deana L. ( June 2019 , Harmful Algae)
5. Dynamics of an intense Alexandrium catenella red tide in the Gulf of Maine: satellite observations and numerical modeling

   https://doi.org/10.1016/j.hal.2020.101927  

   Li, Yizhen ; Stumpf, Richard P. ; McGillicuddy, D.J. ; He, Ruoying ( November 2020 , Harmful Algae)

   null (Ed.)