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Title: Iron–Sulfur Clusters in Zinc Finger Proteins
Zinc finger (ZF) proteins are proteins that use zinc as a structural cofactor. The common feature among all ZFs is that they contain repeats of four cysteine and/or histidine residues within their primary amino acid sequence. With the explosion of genome sequencing in the early 2000s, a large number of proteins were annotated as ZFs based solely upon amino acid sequence. As these proteins began to be characterizedexperimentally, it was discovered that some of these proteins contain iron–sulfur sites either in place of or in addition to zinc. Here, we describe methods to isolate and characterize one such ZF protein, cleavage and polyadenylation specificity factor 30 (CPSF3O) with respect to its metal-loading and RNA-binding activity.  more » « less
Award ID(s):
1708732
NSF-PAR ID:
10065273
Author(s) / Creator(s):
; ;
Date Published:
Journal Name:
Methods in enzymology
Volume:
599
ISSN:
1557-7988
Page Range / eLocation ID:
101-137
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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