An official website of the United States government
ABSTRACT With the advent of ALMA, it is now possible to observationally constrain how discs form around deeply embedded protostars. In particular, the recent ALMA C3H2 line observations of the nearby protostar L1527 have been interpreted as evidence for the so-called ‘centrifugal barrier,’ where the protostellar envelope infall is gradually decelerated to a stop by the centrifugal force in a region of super-Keplerian rotation. To test the concept of centrifugal barrier, which was originally based on angular momentum conserving-collapse of a rotating test particle around a fixed point mass, we carry out simple axisymmetric hydrodynamic simulations of protostellar disc formation including a minimum set of ingredients: self-gravity, rotation, and a prescribed viscosity that enables the disc to accrete. We find that a super-Keplerian region can indeed exist when the viscosity is relatively large but, unlike the classic picture of centrifugal barrier, the infalling envelope material is not decelerated solely by the centrifugal force. The region has more specific angular momentum than its surrounding envelope material, which points to an origin in outward angular momentum transport in the disc (subject to the constraint of disc expansion by the infalling envelope), rather than the spin-up of the envelope material envisioned in the classic picture as it falls closer to the centre in order to conserve angular momentum. For smaller viscosities, the super-Keplerian rotation is weaker or non-existing. We conclude that, despite the existence of super-Keplerian rotation in some parameter regime, the classic picture of centrifugal barrier is not supported by our simulations.
more »
« less
Non-centrifugal microfluidic nucleic acid testing on lab-on-a-disc
Most lab-on-a-disc platforms use centrifugal force to pump liquids. In this work, we demonstrated a novel energy efficient non-centrifugal lab-on-a-disc nucleic acid testing device in which no liquid motion is involved. Streamlined DNA extraction, amplification, and real-time detection on a single microfluidic reagent disc are achieved by actuating the DNA-carrying magnetic beads against stationary reagent droplets. Using malaria spiked whole blood as a testing model, we demonstrated an excellent detection limit of 0.5 parasites/µl, sufficient for detecting asymptomatic malaria parasite carriers.
more »
« less
- Award ID(s):
- 1710831
- PAR ID:
- 10095724
- Date Published:
- Journal Name:
- Transducer 2019
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
More Like this
-
-
Abstract In vitro mechanical testing of intervertebral discs is crucial for basic science and pre‐clinical testing. Generally, these tests aim to replicate in vivo conditions, but simplifications are necessary in specimen preparation and mechanical testing due to complexities in both structure and the loading conditions required to replicate in vivo conditions. There has been a growing interest in developing a consensus of testing protocols within the spine community to improve comparison of results between studies. The objective of this study was to perform axial compression experiments on bovine bone‐disc‐bone specimens at three institutions. No differences were observed between testing environment being air, with PBS soaked gauze, or a PBS bath (P > .206). A 100‐fold increase in loading rate resulted in a small (2%) but significant increase in compressive mechanics (P < .017). A 7% difference in compressive stiffness between Labs B and C was eliminated when values were adjusted for test system compliance. Specimens tested at Lab A, however, were found to be stiffer than specimens from Lab B and C. Even after normalizing for disc geometry and adjusting for system compliance, an ∼35% difference was observed between UK based labs (B and C) and the USA based lab (A). Large differences in specimen stiffness may be due to genetic differences between breeds or in agricultural feed and use of growth hormones; highlighting significant challenges in comparing mechanics data across studies. This research provides a standardized test protocol for the comparison of spinal specimens and provides steps towards understanding how location and test set‐up may affect biomechanical results.more » « less
-
Abstract The interactions of environmental, geographic, socio-demographic, and epidemiological factors in shaping mosquito-borne disease transmission dynamics are complex and changeable, influencing the abundance and distribution of vectors and the pathogens they transmit. In this study, 27 years of cross-sectional malaria survey data (1990–2017) were used to examine the effects of these factors onPlasmodium falciparumandPlasmodium vivaxmalaria presence at the community level in Africa and Asia. Monthly long-term, open-source data for each factor were compiled and analyzed using generalized linear models and classification and regression trees. Both temperature and precipitation exhibited unimodal relationships with malaria, with a positive effect up to a point after which a negative effect was observed as temperature and precipitation increased. Overall decline in malaria from 2000 to 2012 was well captured by the models, as was the resurgence after that. The models also indicated higher malaria in regions with lower economic and development indicators. Malaria is driven by a combination of environmental, geographic, socioeconomic, and epidemiological factors, and in this study, we demonstrated two approaches to capturing this complexity of drivers within models. Identifying these key drivers, and describing their associations with malaria, provides key information to inform planning and prevention strategies and interventions to reduce malaria burden.more » « less
-
Abstract Enzymatic DNA amplification‐based approaches involving intercalating DNA‐binding fluorescent dyes and expensive optical detectors are the gold standard for nucleic acid detection. As components of a simplified and miniaturized system, conventional silicon‐based ion sensitive field effect transistors (ISFETs) that measure a decrease in pH due to the generation of pyrophosphates during DNA amplification have been previously reported. In this article, Bst polymerase in a loop‐mediated isothermal amplification (LAMP) reaction combined with target‐specific primers and crumpled graphene field effect transistors (gFETs) to electrically detect amplification by sensing the reduction in primers is used. Graphene is known to adsorb single‐stranded DNA due to noncovalent π–π bonds, but not double‐stranded DNA. This approach does not require any surface functionalization and allows the detection of primer concentrations at the endpoint of reactions. As recently demonstrated, the crumpled gFET over the conventional flat gFET sensors due to their superior sensitivity is chosen. The endpoint of amplification reaction with starting concentrations down to 8 × 10−21min 90 min including the time of amplification and detection is detected. With its high sensitivity and small footprint, this platform will help bring complex lab‐based diagnostic and genotyping amplification assays to the point‐of‐care.more » « less
- Free Publicly Accessible Full Text
-
Accepted Manuscript1.0
- Conference Paper:
- The DOI is not currently available.
