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Title: Repression of turnip crinkle virus replication by its replication protein p88
We recently reported that p28, one of the two turnip crinkle virus (TCV) replication proteins, trans-complemented a defective TCV lacking p28, yet repressed the replication of another TCV replicon encoding wildtype p28 (Zhang et al., 2017). Here we show that p88, the TCV-encoded RNA-dependent RNA polymerase, likewise trans-complemented a p88-defective TCV replicon, but repressed one encoding wild-type p88. Surprisingly, lowering p88 protein levels enhanced trans-complementation, but weakened repression. Repression by p88 was not simply due to protein over-expression, as deletion mutants missing 127 or 224 N-terminal amino acids accumulated to higher levels but were poor repressors. Finally, both trans-complementation and repression by p88 were accompanied by preferential accumulation of subgenomic RNA2, and a novel class of small TCV RNAs. Our results suggest that repression of TCV replication by p88 may manifest a viral mechanism that regulates the ratio of genomic and subgenomic RNAs based on p88 abundance.  more » « less
Award ID(s):
1758912
PAR ID:
10112999
Author(s) / Creator(s):
; ; ; ;
Date Published:
Journal Name:
Virology
Volume:
526
ISSN:
0042-6822
Page Range / eLocation ID:
165-172
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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