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Title: Inverse scattering for reflection intensity phase microscopy
Reflection phase imaging provides label-free, high-resolution characterization of biological samples, typically using interferometric-based techniques. Here, we investigate reflection phase microscopy fromintensity-only measurements under diverse illumination. We evaluate the forward and inverse scattering model based on the first Born approximation for imaging scattering objects above a glass slide. Under this design, the measured field combineslinearforward-scattering and height-dependentnonlinearback-scattering from the object that complicates object phase recovery. Using only the forward-scattering, we derive a linear inverse scattering model and evaluate this model’s validity range in simulation and experiment using a standard reflection microscope modified with a programmable light source. Our method provides enhanced contrast of thin, weakly scattering samples that complement transmission techniques. This model provides a promising development for creating simplified intensity-based reflection quantitative phase imaging systems easily adoptable for biological research.  more » « less
Award ID(s):
1846784
PAR ID:
10130828
Author(s) / Creator(s):
; ; ; ;
Publisher / Repository:
Optical Society of America
Date Published:
Journal Name:
Biomedical Optics Express
Volume:
11
Issue:
2
ISSN:
2156-7085
Format(s):
Medium: X Size: Article No. 911
Size(s):
Article No. 911
Sponsoring Org:
National Science Foundation
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