Gabor-domain optical coherence microscopy (GDOCM) demonstrated
Two-photon microscope using a fiber-based approach for supercontinuum generation and light delivery to a small-footprint optical head
In this Letter, we report a low-cost, portable, two-photon excitation fluorescence microscopy imager that uses a fiber-based approach for both femtosecond supercontinuum (SC) generation and light delivery to the optical head. The SC generation is based on a tapered polarization-maintaining photonic crystal fiber that uses pre-chirped femtosecond narrowband pulses to generate a coherent SC spectrum with a bandwidth of approximately 300 nm. Using this approach, high-power, near-transform-limited, wavelength-selectable SC pulses are generated and directly delivered to the imaging optical head. Preliminary testing of this imager on brain slices is presented, demonstrating a high signal-to-noise ratio and sub-cellular imaging capabilities to a depth of approximately 200 µm. These results demonstrate the suitability of the technology for
- Award ID(s):
- 1841539
- PAR ID:
- 10133631
- Publisher / Repository:
- Optical Society of America
- Date Published:
- Journal Name:
- Optics Letters
- Volume:
- 45
- Issue:
- 4
- ISSN:
- 0146-9592; OPLEDP
- Format(s):
- Medium: X Size: Article No. 909
- Size(s):
- Article No. 909
- Sponsoring Org:
- National Science Foundation
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