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Title: Nanoscale coupling of endocytic pit growth and stability
Clathrin-mediated endocytosis, an essential process for plasma membrane homeostasis and cell signaling, is characterized by stunning heterogeneity in the size and lifetime of clathrin-coated endocytic pits (CCPs). If and how CCP growth and lifetime are coupled and how this relates to their physiological function are unknown. We combine computational modeling, automated tracking of CCP dynamics, electron microscopy, and functional rescue experiments to demonstrate that CCP growth and lifetime are closely correlated and mechanistically linked by the early-acting endocytic F-BAR protein FCHo2. FCHo2 assembles at the rim of CCPs to control CCP growth and lifetime by coupling the invagination of early endocytic intermediates to clathrin lattice assembly. Our data suggest a mechanism for the nanoscale control of CCP growth and stability that may similarly apply to other metastable structures in cells.  more » « less
Award ID(s):
1900374
PAR ID:
10148664
Author(s) / Creator(s):
; ; ; ; ; ;
Date Published:
Journal Name:
Science Advances
Volume:
5
Issue:
11
ISSN:
2375-2548
Page Range / eLocation ID:
eaax5775
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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