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1. Developability Assessment of an Isolated CH2 Immunoglobulin Domain

   https://doi.org/10.1021/acs.analchem.0c02663  

   Pastrana, B.; Nieves, S.; Li, W.; Liu, X.; Dimitrov, D.S. (December 2020, Analytical chemistry)

   The IgG CH2 domain continues to hold promise for the development of new therapeutic entities due to its bi-functional role as a biomarker and effector protein. The need for further understanding of molecular stability and aggregation in therapeutic proteins has led to the development of a breakthrough quantum cascade laser microscope to allow for real-time comparability assessment of an array of related proteins in solution upon thermal perturbation. Our objective was to perform a comprehensive developability assessment of three similar monoclonal antibody fragments: CH2, CH2s, and m01s. The CH2 construct consists of residues Pro238 to Lys340 of the IgG1 heavy chain sequence. CH2s has a 7-residue deletion at the N-terminus and a 16-residue C-terminal extension containing a histidine tag. The m01s construct is identical to CH2s except for two cysteines introduced at positions 242 and 334. A series of hyperspectral images was acquired during thermal perturbation from 28 to 60 oC for all three proteins in an array. Co-distribution and 2D IR correlation spectroscopies yielded the mechanism of aggregation and stability for these three proteins. The level of detail is unprecedented, identifying the regions within CH2 and CH2s that are prone to self-association and establishing the differences in stability. Furthermore, CH2 helical segments, beta-sheets, beta-turns, and random coil regions were less stable than in CH2s and m01s, due to the presence of the N-terminal 310-helix and beta-turn type III. The engineered disulfide bridge in m01s eliminated the self-association process and rendered this mAb fragment the most stable. 

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2. CH2: A Hybrid Operational/Analytical Processing Benchmark for NoSQL

   https://doi.org/10.1007/978-3-030-94437-7_5  

   Carey, M.; Lychagin, D.; Muralikrishna, M.; Sarawathy, V; Westmann, T. (January 2022, Proc. 13th TPC Technology Conf. on Performance Evaluation & Benchmarking (TPC TC))

   Nambiar, R; Poess, M. (Ed.)

   Database systems with hybrid data management support, referred to as HTAP or HOAP architectures, are gaining popularity. These first appeared in the relational world, and the CH-benCHmark (CH) was proposed in 2011 to evaluate such relational systems. Today, one finds NoSQL database systems gaining adoption for new applications. In this paper we present CH2, a new benchmark – created with CH as its starting point – aimed at evaluating hybrid data platforms in the document data management world. Like CH, CH2 borrows from and extends both TPC-C and TPC-H. Differences from CH include a document-oriented schema, a data generation scheme that creates a TPC-H-like history, and a “do over” of the CH queries that is more in line with TPC-H. This paper details shortcomings that we uncovered in CH, the design of CH2, and preliminary results from running CH2 against Couchbase Server 7.0 (whose Query and Analytics services provide HOAP support for NoSQL data). The results provide insight into the performance isolation and horizontal scalability properties of Couchbase Server 7.0 as well as demonstrating the efficacy of CH2 for evaluating such platforms. 

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3. Reaction of N2O with the prototype singlet biradical CH2: A theoretical study

   https://doi.org/10.1016/j.cplett.2020.137446  

   Nguyen, Thanh Lam; Ravishankara, A.R.; Stanton, John F. (June 2020, Chemical Physics Letters)
4. Gyroscope-like platinum(IV) complexes of the macrocyclic dibridgehead diphosphine P((CH2)14)3P

   https://doi.org/10.1016/j.jorganchem.2023.122789  

   Zhu, Yun; Dey_Baksi, Sourajit; Bhuvanesh, Nattamai; Reibenspies, Joseph H; Gladysz, John A (September 2023, Journal of Organometallic Chemistry)
5. Towards multistranded molecular wires: Syntheses, structures, and reactivities of tetraplatinum bis(polyynediyl) complexes with Pt-Cx-Pt-(P(CH2)3P)2-Pt-Cx-Pt-(P(CH2)3P)2 cores (x = 4, 6, 8)

   https://doi.org/10.1039/c002041a  

   Owen, Gareth R.; Gauthier, Sébastien; Weisbach, Nancy; Hampel, Frank; Bhuvanesh, Nattamai; Gladysz, John A. (January 2010, Dalton Transactions)