More Like this

1. Adaptive responses of marine diatoms to zinc scarcity and ecological implications

   https://doi.org/10.1038/s41467-022-29603-y  

   Kellogg, Riss M.; Moosburner, Mark A.; Cohen, Natalie R.; Hawco, Nicholas J.; McIlvin, Matthew R.; Moran, Dawn M.; DiTullio, Giacomo R.; Subhas, Adam V.; Allen, Andrew E.; Saito, Mak A. (December 2022, Nature Communications)

   Abstract Scarce dissolved surface ocean concentrations of the essential algal micronutrient zinc suggest that Zn may influence the growth of phytoplankton such as diatoms, which are major contributors to marine primary productivity. However, the specific mechanisms by which diatoms acclimate to Zn deficiency are poorly understood. Using global proteomic analysis, we identified two proteins (ZCRP-A/B, Zn/Co Responsive Protein A/B) among four diatom species that became abundant under Zn/Co limitation. Characterization using reverse genetic techniques and homology data suggests putative Zn/Co chaperone and membrane-bound transport complex component roles for ZCRP-A (a COG0523 domain protein) and ZCRP-B, respectively. Metaproteomic detection of ZCRPs along a Pacific Ocean transect revealed increased abundances at the surface (<200 m) where dZn and dCo were scarcest, implying Zn nutritional stress in marine algae is more prevalent than previously recognized. These results demonstrate multiple adaptive responses to Zn scarcity in marine diatoms that are deployed in low Zn regions of the Pacific Ocean. 

   more » « less
2. Potential interactions between diatoms and bacteria are shaped by trace element gradients in the Southern Ocean

   https://doi.org/10.3389/fmars.2022.876830  

   Sterling, Alexa R.; Holland, Laura Z.; Bundy, Randelle M.; Burns, Shannon M.; Buck, Kristen N.; Chappell, P. Dreux; Jenkins, Bethany D. (March 2023, Frontiers in Marine Science)

   The growth of diatoms in the Southern Ocean, especially the region surrounding the West Antarctic Peninsula, is frequently constrained by low dissolved iron and other trace metal concentrations. This challenge may be overcome by mutualisms between diatoms and co-occurring associated bacteria, in which diatoms produce organic carbon as a substrate for bacterial growth, and bacteria produce siderophores, metal-binding ligands that can supply diatoms with metals upon uptake as well as other useful secondary compounds for diatom growth like vitamins. To examine the relationships between diatoms and bacteria in the plankton (diatom) size class (> 3 µm), we sampled both bacterial and diatom community composition with accompanying environmental metadata across a naturally occurring concentration gradient of macronutrients, trace metals and siderophores at 21 stations near the West Antarctic Peninsula (WAP). Offshore Drake Passage stations had low dissolved iron (0.33 ± 0.15 nM), while the stations closer to the continental margin had higher dissolved iron (5.05 ± 1.83 nM). A similar geographic pattern was observed for macronutrients and most other trace metals measured, but there was not a clear inshore-offshore gradient in siderophore concentrations. The diatom and bacteria assemblages, determined using 18S and 16S rDNA sequencing respectively, were similar by location sampled, and variance in both assemblages was driven in part by concentrations of soluble reactive phosphorous, dissolved manganese, and dissolved copper, which were all higher near the continent. Some of the most common diatom sequence types observed were Thalassiosira and Fragilariopsis , and bacteria in the plankton size fraction were most commonly Bacteroidetes and Gammaproteobacteria. Network analysis showed positive associations between diatoms and bacteria, indicating possible in situ mutualisms through strategies such as siderophore and vitamin biosynthesis and exchange. This work furthers the understanding of how naturally occurring gradients of metals and nutrients influence diatom-bacteria interactions. Our data suggest that distinct groups of diatoms and associated bacteria are interacting under different trace metal regimes in the WAP, and that diatoms with different bacterial partners may have different modes of biologically supplied trace metals. 

   more » « less
3. Re‐Evaluating Hydrogen Sulfide as a Sink for Cadmium and Zinc in the Oxic to Suboxic Upper Water Column of the Pacific Ocean

   https://doi.org/10.1029/2023GB007881  

   Buckley, N R; Black, E E; Kenyon, J A; Lanning, N T; Sieber, M; Conway, T M; Fitzsimmons, J N; Cutter, G A (March 2024, Global Biogeochemical Cycles)

   Abstract Hydrogen sulfide is produced by heterotrophic bacteria in anoxic waters and via carbonyl sulfide hydrolysis and phytoplankton emissions under oxic conditions. Apparent losses of dissolved cadmium (dCd) and zinc (dZn) in oxygen minimum zones (OMZs) of the Atlantic and Pacific Oceans have been attributed to metal‐sulfide precipitation formed via dissimilatory sulfate reduction. It has also been argued that such a removal process could be a globally important sink for dCd and dZn. However, our studies from the North Pacific OMZ show that dissolved and particulate sulfide concentrations are insufficient to support the removal of dCd via precipitation. In contrast, apparent dCd and dZn deficits in the eastern tropical South Pacific OMZ do reside in the oxycline with particulate sulfide maxima, but they also coincide with the secondary fluorescence maxima, suggesting that removal via sulfide precipitation may be due to a combination of dissimilatory and assimilatory sulfate reduction. Notably, dCd loss via precipitation with sulfide from assimilatory reduction was found in upper oxic waters of the North Pacific. While dissimilatory sulfate reduction may explain local dCd and dZn losses in some OMZs, our evaluation of North Pacific OMZs demonstrates that dCd and dZn losses are unlikely to be a globally relevant sink. Nevertheless, metal sulfide losses due to assimilatory sulfate reduction in surface waters should be considered in future biogeochemical models of oceanic Cd (and perhaps Zn) cycling. 

   more » « less
4. The acceleration of dissolved cobalt's ecological stoichiometry due to biological uptake, remineralization, and scavenging in the Atlantic Ocean

   https://doi.org/10.5194/bg-14-4637-2017  

   Saito, Mak A.; Noble, Abigail E.; Hawco, Nicholas; Twining, Benjamin S.; Ohnemus, Daniel C.; John, Seth G.; Lam, Phoebe; Conway, Tim M.; Johnson, Rod; Moran, Dawn; et al (January 2017, Biogeosciences)

   The stoichiometry of biological components and their influence on dissolved distributions have long been of interest in the study of the oceans. Cobalt has the smallest oceanic inventory of inorganic micronutrients and hence is particularly vulnerable to influence by internal oceanic processes including euphotic zone uptake, remineralization, and scavenging. Here we observe not only large variations in dCo : P stoichiometry but also the acceleration of those dCo : P ratios in the upper water column in response to several environmental processes. The ecological stoichiometry of total dissolved cobalt (dCo) was examined using data from a US North Atlantic GEOTRACES transect and from a zonal South Atlantic GEOTRACES-compliant transect (GA03/3_e and GAc01) by Redfieldian analysis of its statistical relationships with the macronutrient phosphate. Trends in the dissolved cobalt to phosphate (dCo : P) stoichiometric relationships were evident in the basin-scale vertical structure of cobalt, with positive dCo : P slopes in the euphotic zone and negative slopes found in the ocean interior and in coastal environments. The euphotic positive slopes were often found to accelerate towards the surface and this was interpreted as being due to the combined influence of depleted phosphate, phosphorus-sparing (conserving) mechanisms, increased alkaline phosphatase metalloenzyme production (a zinc or perhaps cobalt enzyme), and biochemical substitution of Co for depleted Zn. Consistent with this, dissolved Zn (dZn) was found to be drawn down to only 2-fold more than dCo, despite being more than 18-fold more abundant in the ocean interior. Particulate cobalt concentrations increased in abundance from the base of the euphotic zone to become  ∼  10 % of the overall cobalt inventory in the upper euphotic zone with high stoichiometric values of  ∼  400 µmol Co mol⁻¹ P. Metaproteomic results from the Bermuda Atlantic Time-series Study (BATS) station found cyanobacterial isoforms of the alkaline phosphatase enzyme to be prevalent in the upper water column, as well as a sulfolipid biosynthesis protein indicative of P sparing. The negative dCo : P relationships in the ocean interior became increasingly vertical with depth, and were consistent with the sum of scavenging and remineralization processes (as shown by their dCo : P vector sums). Attenuation of the remineralization with depth resulted in the increasingly vertical dCo : P relationships. Analysis of particulate Co with particulate Mn and particulate phosphate also showed positive linear relationships below the euphotic zone, consistent with the presence and increased relative influence of Mn oxide particles involved in scavenging. Visualization of dCo : P slopes across an ocean section revealed hotspots of scavenging and remineralization, such as at the hydrothermal vents and below the oxygen minimum zone (OMZ) region, respectively, while that of an estimate of Co* illustrated stoichiometrically depleted values in the mesopelagic and deep ocean due to scavenging. This study provides insights into the coupling between the dissolved and particulate phase that ultimately creates Redfield stoichiometric ratios, demonstrating that the coupling is not an instantaneous process and is influenced by the element inventory and rate of exchange between phases. Cobalt's small water column inventory and the influence of external factors on its biotic stoichiometry can erode its limited inertia and result in an acceleration of the dissolved stoichiometry towards that of the particulate phase in the upper euphotic zone. As human use of cobalt grows exponentially with widespread adoption of lithium ion batteries, there is a potential to affect the limited biogeochemical inertia of cobalt and its resultant ecology in the oceanic euphotic zone. 

   more » « less
5. Co-occurrence and successional patterns among diatoms, dinoflagellates, and potential parasites in a coastal upwelling experiment

   Vineis, J H; Burger, J M; Fawcett, S E; Ward, B B (April 2025, Limnology and oceanography)

   Diatom-dominated blooms in coastal upwelling systems contribute disproportionately to global primary production. The fate of carbon captured during a diatom bloom is often influenced by species-specific ecological differences. However, successional patterns that take place during a diatom bloom are often oversimplified, and the diversity of diatom adaptations to different stages of a bloom remains poorly characterized. To improve our understanding of diatom specificity to certain conditions within a bloom, we employed microscopy, 18S rRNA amplicons, and biogeochemical analysis within a simulated upwelling mesocosm experiment. We successfully simulated a diatom bloom and found that diatoms bloomed during early and late phases of the bloom. Surprisingly, the relative abundance of congeneric diatoms with the Thalassiosira, Chaetoceros, and Pseudonitzschia displayed opposing patterns that were consistent among experimental mesocosms. The late stage of the bloom was especially interesting because some diatoms continued to bloom among mixotrophic dinoflagellate genera Akashiwo, Heterocapsa, and Prorocentrum. Additionally, Syndiniales putative parasites were correlated with several diatoms, especially in the initial phase of the bloom. The novel observations of consistent rapid successional changes within our mesocosms reflect the ability of diatom and dinoflagellate genera to occupy bloom conditions that fall outside traditional expectations. Syndiniales parasite co-occurrence with blooming diatoms may be important to successional trends of coastal diatom populations, and this parasitic interaction deserves further study in coastal upwelling systems. This study indicates there are underlying diatom traits and biotic interactions that should be considered when estimating their contribution to productivity and carbon cycling within upwelling systems. 

   more » « less