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Title: Electron Paramagnetic Resonance as a Tool for Studying Membrane Proteins
Membrane proteins possess a variety of functions essential to the survival of organisms. However, due to their inherent hydrophobic nature, it is extremely difficult to probe the structure and dynamic properties of membrane proteins using traditional biophysical techniques, particularly in their native environments. Electron paramagnetic resonance (EPR) spectroscopy in combination with site-directed spin labeling (SDSL) is a very powerful and rapidly growing biophysical technique to study pertinent structural and dynamic properties of membrane proteins with no size restrictions. In this review, we will briefly discuss the most commonly used EPR techniques and their recent applications for answering structure and conformational dynamics related questions of important membrane protein systems.  more » « less
Award ID(s):
1807131 1725502
PAR ID:
10181482
Author(s) / Creator(s):
;
Date Published:
Journal Name:
Biomolecules
Volume:
10
Issue:
5
ISSN:
2218-273X
Page Range / eLocation ID:
763
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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