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  • ELVIS: A Correlated Light-Field and Digital Holographic Microscope for Field and Laboratory Investigations – Field Demonstration
Title: ELVIS: A Correlated Light-Field and Digital Holographic Microscope for Field and Laboratory Investigations – Field Demonstration
Following the previous article, here we describe the first field demonstration of the ELVIS system, performed at Newport Beach, CA. We examined ocean water to detect microorganisms using the combined holographic and light-field fluorescence microscope and successfully detected both eukaryotes and prokaryotes. The shared field of view provided simultaneous bright-field (amplitude), phase, and fluorescence information from both chlorophyll autofluorescence and acridine orange staining. The entire process was performed in a nearly autonomous manner using a specifically designed sample processing unit (SPU) and custom acquisition software. We also discuss improvements to the system made after the field test that will make it more broadly useful to other types of fluorophores and samples.  more » « less
Award ID(s):
1828793
PAR ID:
10185653
Author(s) / Creator(s):
; ; ; ; ; ; ; ;
Date Published:
Journal Name:
Microscopy Today
Volume:
28
Issue:
4
ISSN:
1551-9295
Page Range / eLocation ID:
14 to 18
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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