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Title: Strategies for in vitro engineering of the translation machinery
Abstract Engineering the process of molecular translation, or protein biosynthesis, has emerged as a major opportunity in synthetic and chemical biology to generate novel biological insights and enable new applications (e.g. designer protein therapeutics). Here, we review methods for engineering the process of translation in vitro. We discuss the advantages and drawbacks of the two major strategies—purified and extract-based systems—and how they may be used to manipulate and study translation. Techniques to engineer each component of the translation machinery are covered in turn, including transfer RNAs, translation factors, and the ribosome. Finally, future directions and enabling technological advances for the field are discussed.  more » « less
Award ID(s):
1716766
NSF-PAR ID:
10187257
Author(s) / Creator(s):
; ;
Date Published:
Journal Name:
Nucleic Acids Research
Volume:
48
Issue:
3
ISSN:
0305-1048
Page Range / eLocation ID:
1068 to 1083
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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