More Like this

1. Rhizobiome of ‘Ōhi‘a Lehua (Metrosideros polymorpha) Offers Insight into Plant-Microbe-Invertebrate Interactions in the Subsurface

   https://doi.org/10.3897/aca.6.e108263  

   Engel, Annette ; Steck, Mireille ; Paterson, Audrey ; Van Gieson, Amir ; Porter, Megan ; Chong, Rebecca ( October 2023 , ARPHA Conference Abstracts)

   Chi Fru, Ernest ; Chik, Alex ; Colwell, Fredrick ; Dittrich, Maria ; Engel, Annette ; Keenan, Sarah ; Meckenstock, Rainer ; Omelon, Christopher ; Purkamo, Lotta ; Weisener, Chris (Ed.)

   Roots are common features in basaltic lava tube caves on the island of Hawai‘i. For the past 50 years, new species of cave-adapted invertebrates, including cixiid planthoppers, crickets, thread-legged bugs, and spiders, have been discovered from root patches in lava tubes on different volcanoes and across variable climatic conditions. Assessing vegetation on the surface above lava tube passages, as well as genetic characterization of roots from within lava tubes, suggest that most roots belong to the native pioneer tree, ‘ōhi‘a lehua (Metrosideros polymorpha). Planthoppers are the primary consumers of sap at the base of the subsurface food web. However, root physicochemistry and rhizobiome microbial diversity and functional potential have received little attention. This study focuses on characterizing the ‘ōhi‘a rhizobiome, accessed from free-hanging roots inside lava tubes. Using these results, we can begin to evaluate the development and evolution of plant-microbe-invertebrate relationships.

   We explored lava tubes formed in flows of differing elevations and ages, from about 140 to 3000 years old, on Mauna Loa, Kīlauea, and Hualālai volcanoes on Hawai‘i Island. Invertebrate diversity was evaluated from root galleries and non-root galleries, in situ fluid physicochemistry was measured, and root and bare rock fluids (e.g., water, sap) were collected to determine major ion concentrations, as well as non-purgeable organic carbon (NPOC) and total nitrogen (TN) content. To verify root identity, DNA was extracted, and three sets of primers were used. After screening for onlyMetrosiderosspp., the V4 region of the 16S rRNA gene was sequenced and taxonomy was assigned.

   Root fluids were viscous and ranged in color from clear to yellow to reddish orange. Root fluids had 2X to 10X higher major ion concentrations compared to rock water. The average root NPOC and TN concentrations were 192 mg/L and 5.2 mg/L, respectively, compared to rock water that had concentrations of 6.8 mg/L and 1.8 mg/L, respectively. Fluids from almost 300 root samples had pH values that ranged from 2.2 to 5.6 (average pH 4.63) and were lower than rock water (average pH 6.39). Root fluid pH was comparable to soil pH from montane wet forests dominated by ‘ōhi‘a (Selmants et al. 2016), which can grow in infertile soil with pH values as low as 3.6. On Hawai‘i, rain water pH averages 5.2 at sea level and systematically decreases with elevation to pH 4.3 at 2500 m (Miller and Yoshinaga 2012), but root fluid pH did not correlate with elevation, temperature, relative humidity, inorganic and organic constituents, or age of flow. Root fluid acidity is likely due to concentrated organic compounds, sourced as root exudates, and this habitat is acidic for the associated invertebrates.

   From 62 root samples, over 66% were identified to the genusMetrosideros. A few other identifications of roots from lava tube systems where there had been extensive clear-cutting and ranching included monkey pod tree, coconut palm,Ficusspp., and silky oak.

   The 16S rRNA gene sequence surveys revealed that root bacterial communities were dominated by few groups, including Burkholderiaceae, as well as Acetobacteraceae, Sphingomonadaceae, Acidobacteriaceae, Gemmataceae, Xanthobacteraceae, and Chitinophagaceae. However, most of the reads could not be classified to a specific genus, which suggested that the rhizobiome harbor novel diversity. Diversity was higher from wetter climates. The root communities were distinct from those described previously from ‘ōhi‘a flowers and leaves (Junker and Keller 2015) and lava tube rocky surfaces (Hathaway et al. 2014) where microbial groups were specifically presumed capable of heterotrophy, methanotrophy, diazotrophy, and nitrification. Less can be inferred for the rhizobiome metabolism, although most taxa are likely aerobic heterotrophs. Within the Burkholderiaceae, there were high relative abundances of sequences affiliated with the genusParaburkholderia, which includes known plant symbionts, as well as the acidophilic generaAcidocellaandAcidisomafrom the Acetobacteraceae, which were retrieved predominately from caves in the oldest lava flows that also had the lowest root pH values. It is likely that the bacterial groups are capable of degrading exudates and providing nutritional substrates for invertebrate consumers that are not provided by root fluids (i.e., phloem) alone.

   As details about the biochemistry of ‘ōhi‘a have been missing, characterizing the rhizobiome from lava tubes will help to better understand potential plant-microbe-invertebrate interactions and ecological and evolutionary relationships through time. In particular, the microbial rhizobiome may produce compounds used by invertebrates nutritionally or that affect their behavior, and changes to the rhizobiome in response to environmental conditions may influence invertebrate interactions with the roots, which could be important to combat climate change effects or invasive species introductions.

    

   more » « less
2. Shade is the most important factor limiting growth of a woody range expander

   https://doi.org/10.1371/journal.pone.0242003  

   Ward, David ( December 2020 , PLOS ONE)

   Gomory, Dusan (Ed.)

   The expansion of woody plants into grasslands and old fields is often ascribed to fire suppression and heavy grazing, especially by domestic livestock. However, it is also recognized that nutrient availability and interspecific competition with grasses and other woody plants play a role in certain habitats. I examined potential factors causing range- and niche expansion by the eastern redcedar Juniperus virginiana , the most widespread conifer in the eastern United States, in multifactorial experiments in a greenhouse. Historical records suggest that the eastern redcedar is a pioneer forest species, and may be replaced as the forest increases in tree density due to shading. Another possible factor that affects its distribution may be nutrient availability, which is higher in old fields and other disturbed lands than in undisturbed habitats. In its historic range, eastern redcedars are particularly abundant on limestone outcrops, often termed ‘cedar barrens’. However, the higher abundance on limestone could be due to reduced interspecific competition rather than a preference for high pH substrates. I manipulated shade, fertilization, lime, and interspecific competition with a common dominant tree, the post oak Quercus stellata . In a separate experiment, I manipulated fire and grass competition. I measured growth rates (height and diameter) and above- and belowground biomass at the end of both experiments. I also measured total non-structural carbohydrates and nitrogen in these plants. Shade was the most important factor limiting the growth rates and biomass of eastern redcedars. I also found that there were significant declines in nitrogen and non-structural carbohydrates when shaded. These results are consistent with the notion that the eastern redcedar is a pioneer forest species, and that shade is the reason that these redcedars are replaced by other tree species. In the second experiment, I found that a single fire had a negative effect on young trees. There was no significant effect of competition with grass, perhaps because the competitive effect was shading by grasses and not nutrient depletion. Overall, the effects of shade were far more apparent than the effects of fire. 

   more » « less
3. White oak and red maple foliar chemistry of urban and reference forests of the eastern US

   https://doi.org/10.6073/pasta/0b48d8744f971ef244a30425fbec15fb  

   Sonti, Nancy F ; Hallett, Richard A. ; Griffin, Kevin L. ; Sullivan, Joe H. ( January 2020 , Environmental Data Initiative)

   Foliar chemistry values were obtained from two important native tree species (white oak (Quercus alba L.) and red maple (Acer rubrum L.)) across urban and reference forest sites of three major cities in the eastern United States during summer 2015 (New York, NY (NYC); Philadelphia, PA; and Baltimore, MD). Trees were selected from secondary growth oak-hickory forests found in New York, NY; Philadelphia, PA; and Baltimore, MD, as well as at reference forest sites outside each metropolitan area. In all three metropolitan areas, urban forest patches and references forest sites were selected based on the presence of red maple and white oak canopy dominant trees in patches of at least 1.5 hectares with slopes less than 25%, and well-drained soils of similar soil series within each metropolitan area. Within each city, several forest patches were selected to capture the variation in forest patch site conditions across an individual city. All reference sites were located in protected areas outside of the city and within intermix wildland-urban interface landscapes, in order to target similar contexts of surrounding land use and population density (Martinuzzi et al. 2015). Several reference sites were selected for each city, located within the same protected area considered representative of rural forests of the region. White oaks were at least 38.1 cm diameter at breast height (DBH), red maples were at least 25.4 cm DBH, and all trees were dominant or co-dominant canopy trees. The trees had no major trunk cavities and had crown vigor scores of 1 or 2 (less than 25% overall canopy damage; Pontius & Hallett 2014). From early July to early August 2015, sun leaves were collected from the periphery of the crown of each tree with either a shotgun or slingshot for subsequent analysis to determine differences in foliar chemistry across cities and urban vs. reference forest site types. The data were used to invstigate whether differences in native tree physiology occur between urban and reference forest patches, and whether those differences are site- and species-specific. A complete analysis of these data can be found in: Sonti, NF. 2019. Ecophysiological and social functions of urban forest patches. Ph.D. dissertation. University of Maryland, College Park, MD. 166 p. References: Martinuzzi S, Stewart SI, Helmers DP, Mockrin MH, Hammer RB, Radeloff VC. 2015. The 2010 wildland-urban interface of the conterminous United States. Research Map NRS-8. US Department of Agriculture, Forest Service, Northern Research Station: Newtown Square, PA. Pontius J, Hallett R. 2014. Comprehensive methods for earlier detection and monitoring of forest decline. Forest Science 60(6): 1156-1163. 

   more » « less
4. Tree density influences ecohydrological drivers of plant–water relations in a larch boreal forest in Siberia

   https://doi.org/10.1002/eco.2132  

   Kropp, Heather ; Loranty, Michael M. ; Natali, Susan M. ; Kholodov, Alexander L. ; Alexander, Heather D. ; Zimov, Nikita S. ; Mack, Michelle C. ; Spawn, Seth A. ( July 2019 , Ecohydrology)

   Transpiration and stomatal conductance in deciduous needleleaf boreal forests of northern Siberia can be highly sensitive to water stress, permafrost thaw, and atmospheric dryness. Additionally, north‐eastern Siberian boreal forests are fire‐driven, and larch (Larixspp.) are the sole tree species. We examined differences in tree water use, stand characteristics, and stomatal responses to environmental drivers between high and low tree density stands that burned 76 years ago in north‐eastern Siberia. Our results provide process‐level insight to climate feedbacks related to boreal forest productivity, water cycles, and permafrost across Arctic regions. The high density stand had shallower permafrost thaw depths and deeper moss layers than the low density stand. Rooting depths and shallow root biomass were similar between stands. Daily transpiration was higher on average in the high‐density stand 0.12 L m⁻² day⁻¹(SE: 0.004) compared with the low density stand 0.10 L m⁻² day⁻¹(SE: 0.001) throughout the abnormally wet summer of 2016. Transpiration rates tended to be similar at both stands during the dry period in 2017 in both stands of 0.10 L m⁻² day⁻¹(SE: 0.002). The timing of precipitation impacted stomatal responses to environmental drivers, and the high density stand was more dependent on antecedent precipitation that occurred over longer periods in the past compared with the low density stand. Post‐fire tree density differences in plant–water relations may lead to different trajectories in plant mortality, water stress, and ecosystem water cycles across Siberian landscapes.

    

   more » « less
5. Eastern US deciduous tree species respond dissimilarly to declining soil moisture but similarly to rising evaporative demand

   https://doi.org/10.1093/treephys/tpaa153  

   Denham, Sander O ; Oishi, A Christopher ; Miniat, Chelcy F ; Wood, Jeffrey D ; Yi, Koong ; Benson, Michael C ; Novick, Kimberly A ( November 2020 , Tree Physiology)

   Whitehead, David (Ed.)

   Abstract Hydraulic stress in plants occurs under conditions of low water availability (soil moisture; θ) and/or high atmospheric demand for water (vapor pressure deficit; D). Different species are adapted to respond to hydraulic stress by functioning along a continuum where, on one hand, they close stomata to maintain a constant leaf water potential (ΨL) (isohydric species), and on the other hand, they allow ΨL to decline (anisohydric species). Differences in water-use along this continuum are most notable during hydrologic stress, often characterized by low θ and high D; however, θ and D are often, but not necessarily, coupled at time scales of weeks or longer, and uncertainty remains about the sensitivity of different water-use strategies to these variables. We quantified the effects of both θ and D on canopy conductance (Gc) among widely distributed canopy-dominant species along the isohydric–anisohydric spectrum growing along a hydroclimatological gradient. Tree-level Gc was estimated using hourly sap flow observations from three sites in the eastern United States: a mesic forest in western North Carolina and two xeric forests in southern Indiana and Missouri. Each site experienced at least 1 year of substantial drought conditions. Our results suggest that sensitivity of Gc to θ varies across sites and species, with Gc sensitivity being greater in dry than in wet sites, and greater for isohydric compared with anisohydric species. However, once θ limitations are accounted for, sensitivity of Gc to D remains relatively constant across sites and species. While D limitations to Gc were similar across sites and species, ranging from 16 to 34% reductions, θ limitations to Gc ranged from 0 to 40%. The similarity in species sensitivity to D is encouraging from a modeling perspective, though it implies that substantial reduction to Gc will be experienced by all species in a future characterized by higher D. 

   more » « less