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1. Consistent shifts in pollinator-relevant floral coloration along Rocky Mountain elevation gradients

   Gray M, Stansberry M ( January 2018 , Journal of ecology)

   Abstract1. Floral colour is a fundamental signal that shapes plant–pollinator interactions. Despite theoretical reasons why floral colours might shift in representation along biotic and abiotic gradients, few studies have examined community-level shifts in colour, and even fewer significant patterns have been detected.2. We examined floral colour on six replicated transects spanning 1,300 m in the Rocky Mountains of Colorado, USA. Along these transects, there is a hypothe-sized shift from bee-dominated to fly-dominated pollination with increasing eleva-tion. The reflectance of flowers of 110 forb and shrub species was measured using a spectrophotometer, and was used to estimate three components of colour (hue, saturation and brightness) in relevant pollinator visual spaces. Percent cover data were collected from 67 sites and used to obtain community-weighted mean (cwm) estimates of floral colour.3. We found strong patterns of elevational change in floral colour. Reflectancecwm of shorter wavelengths (UVB through human blue, 300–500 nm) generally de-creased linearly with elevation, while reflectancecwm of longer wavelengths (human green through red, 500–700 nm) showed hump-shaped patterns with highest reflectance at intermediate elevations. With respect to pollinators, satura-tioncwm increased significantly with elevation in both bee and fly visual spaces, while brightness contrastcwm showed a hump-shaped pattern in bee space and a decline with elevation in fly visual space. For hue, cover of species perceived as bee-blue declined with elevation, while cover of bee-UV-green species showed a hump-shaped pattern. In comparison, we detected no elevational shifts in floral hues as perceived by flies.4. Synthesis. Hue patterns are consistent with the hypothesis that bee pollinators have shaped the geography of floral colour. The roles of fly pollinators and of abi-otic drivers are more difficult to infer, although the drop in floral brightness at high elevations is consistent with predictions that low temperatures and more intense ultraviolet radiation should favour increased pigment concentrations there. Our results indicate that floral colour can be dynamic yet predictable across the land-scape, a pattern that provides opportunities to tease apart the ecological and evo-lutionary drivers of this important plant trait. 

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2. Honest floral signalling traits vary across and within populations in an insect‐pollinated plant

   https://doi.org/10.1111/1365-2435.14405  

   Eisen, Katherine E. ; Boutsi, Sotiria ; Halley, John M. ; Pace, Loretta ; Petrén, Hampus ; Thosteman, Hanna ; Friberg, Magne ( July 2023 , Functional Ecology)

   In flowering plants that produce concealed rewards, pollinator foraging preferences may select for floral advertisement traits that are correlated with rewards. To date, studies have not focused on the potential for honest signals to vary across populations, which could occur due to differences in pollinator communities or plant mating system.

   We tested for variation in honest signals across and within populations and mating systems inArabis alpina, a broadly distributed arctic‐alpine perennial herb that is visited by a variable community of insects. In a greenhouse common garden, we tested for correlations between corolla area, floral scent and nectar volume in 29 populations. In 12 field populations, we examined variation in pollen limitation and corolla area.

   Across and within populations and mating systems, larger flowers generally produced more nectar. Total scent emission was not correlated with nectar production, but two compounds—phenylacetaldehyde and benzyl alcohol—may be honest signals in some populations. Corolla area was correlated with pollen limitation only across populations.

   Our results suggest that honest signals may be similar across populations but may not result from contemporary direct selection on floral advertisements.

   Read the freePlain Language Summaryfor this article on the Journal blog.

    

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3. Scented nectar and the challenge of measuring honest signals in pollination

   https://doi.org/10.1111/1365-2745.13432  

   Burdon, Rosalie C. F. ; Raguso, Robert A. ; Gegear, Robert J. ; Pierce, Ellen C. ; Kessler, André ; Parachnowitsch, Amy L. ; Whitney, ed., Kenneth ( June 2020 , Journal of Ecology)

   Nectar scents are thought to function as honest signals of reward used by pollinators, but this hypothesis has rarely been tested.

   UsingPenstemon digitalis, we examined honest signalling of the nectar volatile (S)‐(+)‐linalool and pollinator responses to linalool in both field and laboratory settings. Because our previous work showed that linalool emission was associated with higher female fitness and that nectar is scented with linalool, we hypothesized that linalool was an honest signal of nectar reward. To assess honesty, we measured linalool–nectar associations including nectar volume, sugar amount, concentration and production rate for inflorescences and flowers in several populations. We also assessed whetherBombus impatiens, the main pollinator ofP. digitalisat our sites, can use linalool as a foraging signal. We supplemented real or artificial flowers in the field and laboratory with varying linalool–nectar combinations to measure pollinator behavioural responses.

   We found that an inflorescence's linalool emissions could be used to predict nectar rewards inP. digitalis, but this was driven by indirect associations with display size rather than directly advertising more profitable flowers. For flowers within inflorescences there was also no evidence for an association between signal and reward. Field tests of bumblebee behaviour were inconclusive. However, in laboratory assays, bumblebees generally used variation in linalool emissions to choose more profitable flowers, demonstrating they can detect differences in linalool emitted byP. digitalisand associate them with reward profitability. These results suggest experiments that decouple display size, scent and reward are necessary to assess whether (and when) bees prefer higher linalool emissions. Bees preferred nectars with lower linalool concentrations when linalool flavoured the nectar solution, suggesting the potential for conflicting pressures on scent emission in the field.

   Synthesis. Our results highlight the challenges of assessing function for traits important to fitness and suggest that the perception of floral signalling honesty may depend on whether pollinators use inflorescences or flowers within inflorescences when making foraging decisions. We conclude that future research on honest signalling in flowering plants, as well as its connection to phenotypic selection, should explicitly define honesty, in theoretical and experimental contexts.

    

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4. A few essential genetic loci distinguish Penstemon species with flowers adapted to pollination by bees or hummingbirds

   https://doi.org/10.1371/journal.pbio.3002294  

   Wessinger, Carolyn A. ; Katzer, Amanda M. ; Hime, Paul M. ; Rausher, Mark D. ; Kelly, John K. ; Hileman, Lena C. ( September 2023 , PLOS Biology)

   Barton, Nick H. (Ed.)

   In the formation of species, adaptation by natural selection generates distinct combinations of traits that function well together. The maintenance of adaptive trait combinations in the face of gene flow depends on the strength and nature of selection acting on the underlying genetic loci. Floral pollination syndromes exemplify the evolution of trait combinations adaptive for particular pollinators. The North American wildflower genusPenstemondisplays remarkable floral syndrome convergence, with at least 20 separate lineages that have evolved from ancestral bee pollination syndrome (wide blue-purple flowers that present a landing platform for bees and small amounts of nectar) to hummingbird pollination syndrome (bright red narrowly tubular flowers offering copious nectar). Related taxa that differ in floral syndrome offer an attractive opportunity to examine the genomic basis of complex trait divergence. In this study, we characterized genomic divergence among 229 individuals from aPenstemonspecies complex that includes both bee and hummingbird floral syndromes. Field plants are easily classified into species based on phenotypic differences and hybrids displaying intermediate floral syndromes are rare. Despite unambiguous phenotypic differences, genome-wide differentiation between species is minimal. Hummingbird-adapted populations are more genetically similar to nearby bee-adapted populations than to geographically distant hummingbird-adapted populations, in terms of genome-wided_XY. However, a small number of genetic loci are strongly differentiated between species. These approximately 20 “species-diagnostic loci,” which appear to have nearly fixed differences between pollination syndromes, are sprinkled throughout the genome in high recombination regions. Several map closely to previously established floral trait quantitative trait loci (QTLs). The striking difference between the diagnostic loci and the genome as whole suggests strong selection to maintain distinct combinations of traits, but with sufficient gene flow to homogenize the genomic background. A surprisingly small number of alleles confer phenotypic differences that form the basis of species identity in this species complex.

    

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5. Data from: A few essential genetic loci distinguish Penstemon species with flowers adapted to pollination by bees or hummingbirds

   https://doi.org/10.5061/dryad.xpnvx0kmp  

   Wessinger, Carolyn ( January 2023 , Dryad)

   In the formation of species, adaptation by natural selection generates distinct combinations of traits that function well together. The maintenance of adaptive trait combinations in the face of gene flow depends on the strength and nature of selection acting on the underlying genetic loci. Floral pollination syndromes exemplify the evolution of trait combinations adaptive for particular pollinators. The North American wildflower genus Penstemon displays remarkable floral syndrome convergence, with at least 20 separate lineages that have evolved from ancestral bee pollination syndrome (wide blue-purple flowers that present a landing platform for bees and small amounts of nectar) to hummingbird pollination syndrome (bright red narrowly tubular flowers offering copious nectar). Related taxa that differ in floral syndrome offer an attractive opportunity to examine the genomic basis of complex trait divergence. In this study, we characterized genomic divergence among 229 individuals from a Penstemon species complex that includes both bee and hummingbird floral syndromes. Field plants are easily classified into species based on phenotypic differences and hybrids displaying intermediate floral syndromes are rare. Despite unambiguous phenotypic differences, genomewide differentiation between species is minimal. Hummingbird-adapted populations are more genetically similar to nearby bee-adapted populations than to geographically distant hummingbird-adapted populations, in terms of genomewide dXY. However, a small number of genetic loci are strongly differentiated between species. These ~ 20 "species-diagnostic loci", which appear to have nearly fixed differences between pollination syndromes, are sprinkled throughout the genome in high recombination regions. Several map closely to previously established floral trait QTLs. The striking difference between the diagnostic loci and the genome as whole suggests strong selection to maintain distinct combinations of traits, but with sufficient gene flow to homogenize the genomic background. A surprisingly small number of alleles confer phenotypic differences that form the basis of species identity in this species complex. 

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