A spectroelectrochemical (SEC) assay for uric acid in human urine has been developed that is essentially reagent‐free and involves relatively short analysis times of ∼12 min per specimen using a single‐point standard addition approach. The SEC method was demonstrated to be selective for uric acid in human urine, avoiding interference through both the usual control parameters (electrolysis potential and measurement wavelength) and the relatively slow kinetics of a post‐electrolysis reaction involving the oxidation product. Results from the SEC analysis of a split set of human urine specimens were well correlated to those obtained using a standard high‐performance liquid chromatography method, suggesting the feasibility of clinical applications.
more » « less- NSF-PAR ID:
- 10238760
- Publisher / Repository:
- Wiley Blackwell (John Wiley & Sons)
- Date Published:
- Journal Name:
- Electroanalysis
- Volume:
- 33
- Issue:
- 1
- ISSN:
- 1040-0397
- Page Range / eLocation ID:
- p. 75-80
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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