Feeding for most animals involves bouts of active ingestion alternating with bouts of no ingestion. In insects, the temporal patterning of bouts varies widely with resource quality and is known to affect growth, development time, and fitness. However, the precise impacts of resource quality and feeding behavior on insect life history traits are poorly understood. To explore and better understand the connections between feeding behavior, resource quality, and insect life history traits, we combined laboratory experiments with a recently proposed mechanistic model of insect growth and development for a larval herbivore,
Conventional and Organic Wheat Germ Have Distinct Physiological Effects in the Tobacco Hornworm, Manduca Sexta: Use of Black Mutant Assay to Detect Environmental Juvenoid Activity of Insect Growth Regulators
Stored grains used in artificial diets are often treated with insecticides to control infestation by pests. In recent years, insect growth regulators (IGRs) have become an increasingly popular form of insect pest control in agricultural settings. Most IGRs specifically target insects by either disrupting their endocrine system or their chitin synthesis. One type of IGRs comprises of chemical analogs of juvenile hormone (JH), a major hormone involved in growth and development of insects. Here we demonstrate that conventional wheat germ contains JH activity and impacts growth and development of the tobacco hornworm, Manduca sexta . Feeding diet containing conventional wheat germ delayed the timing of metamorphosis in wildtype larvae by extending the duration of the final instar. Diet with conventional wheat germ also inhibited melanization of the black mutant larvae and induced the expression of the JH response gene, Krüppel homolog 1 . We demonstrate that the black mutant bioassay is a sensitive assay that can determine the amount of JH activity in stored grains and suggest that this assay may offer a quick and reliable assay to determine the amount of environmental juvenoids. Researchers are urged to use caution when purchasing stored grains for mass-rearing of research insects.
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- Award ID(s):
- 2002354
- NSF-PAR ID:
- 10299937
- Date Published:
- Journal Name:
- Frontiers in Insect Science
- Volume:
- 1
- ISSN:
- 2673-8600
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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- Free Publicly Accessible Full Text
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Accepted Manuscript1.0
- Journal Article:
- https://doi.org/10.3389/finsc.2021.744847
