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Title: Protocol for resolving enzyme orientation and dynamics in advanced porous materials via SDSL-EPR
Enzyme encapsulation in metal-organic frameworks (MOFs)/covalent-organic frameworks (COFs) provides advancement in biocatalysis, yet the structural basis underlying the catalytic performance is challenging to probe. Here, we present an effective protocol to determine the orientation and dynamics of enzymes in MOFs/COFs using site-directed spin labeling and electron paramagnetic resonance spectroscopy. The protocol is demonstrated using lysozyme and can be generalized to other enzymes.  more » « less
Award ID(s):
1942596
NSF-PAR ID:
10300682
Author(s) / Creator(s):
; ; ; ; ; ; ; ;
Date Published:
Journal Name:
STAR protocols
Volume:
2
ISSN:
2666-1667
Page Range / eLocation ID:
100676
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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