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This content will become publicly available on September 17, 2022

Title: Protocol for resolving enzyme orientation and dynamics in advanced porous materials via SDSL-EPR
Enzyme encapsulation in metal-organic frameworks (MOFs)/covalent-organic frameworks (COFs) provides advancement in biocatalysis, yet the structural basis underlying the catalytic performance is challenging to probe. Here, we present an effective protocol to determine the orientation and dynamics of enzymes in MOFs/COFs using site-directed spin labeling and electron paramagnetic resonance spectroscopy. The protocol is demonstrated using lysozyme and can be generalized to other enzymes.
Authors:
; ; ; ; ; ; ; ;
Award ID(s):
1942596
Publication Date:
NSF-PAR ID:
10300682
Journal Name:
STAR protocols
Volume:
2
Page Range or eLocation-ID:
100676
ISSN:
2666-1667
Sponsoring Org:
National Science Foundation
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