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Title: Protocol for resolving enzyme orientation and dynamics in advanced porous materials via SDSL-EPR
Enzyme encapsulation in metal-organic frameworks (MOFs)/covalent-organic frameworks (COFs) provides advancement in biocatalysis, yet the structural basis underlying the catalytic performance is challenging to probe. Here, we present an effective protocol to determine the orientation and dynamics of enzymes in MOFs/COFs using site-directed spin labeling and electron paramagnetic resonance spectroscopy. The protocol is demonstrated using lysozyme and can be generalized to other enzymes.  more » « less
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STAR protocols
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Medium: X
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National Science Foundation
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