Light microscopy provides a window into another world that is not visible to the unaided eye. Because of this and its importance in biological discoveries, the light microscope is an essential tool for scientific studies. It can also be used with a variety of easily obtained specimens to provide dramatic demonstrations of previously unknown features of common plants and animals. Thus, one way to interest young people in science is to start with an introduction to light microscopy. This is an especially effective strategy for individuals who attend less advantaged or under-resourced schools, as they may not have been previously exposed to scientific concepts in their classes. However, introducing light microscopy lessons in the classroom can be challenging because of the high cost of light microscopes, even those that are relatively basic, in addition to their usual large size.
Efforts are underway by our laboratory in collaboration with the Biophysical Society (BPS) to introduce young people to light microscopy using small, easy-to-assemble wooden microscopes developed by Echo Laboratories. The microscopes are available online as low-cost kits ($10 each with shipping), each consisting of 19 parts printed onto an 81⁄2 x 11 inch sheet of light-weight wood (Fig. 1). After punching out the pieces, they can be assembled into a microscope with a moveable stage and a low-power lens, also provided in the kit (Fig. 2). Photos taken with a cell phone through the microscope lens can give magnifications of ~16-18x, or higher. At these magnifications, features of specimens that are not visible to the unaided eye can be easily observed, e.g., small hairs on the margins of leaves or lichens [1]. As a member of the BPS Education Committee, one of us (SAE) wrote a Lesson Plan on Light Microscopy specifically for use with the wooden microscopes. SAE was also able to obtain a gift of 500 wooden microscope kits for the BPS from Echo Laboratories and Chroma Technology Corp in 2016. The wooden microscope kits, together with the lesson plan, have provided the materials for our present outreach efforts.
Rather than giving out the wooden microscope kits to individuals, the BPS asked the Education Committee to maximize the impact of the gift by distributing the microscopes with the Lesson Plan on Light Microscopy to teachers, e.g., through teachers’ workshops or outreach sessions. This strategy was devised to enable the Society to reach a larger number of young people than by giving the microscopes to individuals.
The Education Committee first evaluated the microscopes as a tool to introduce students to scientific concepts by providing microscopes to a BPS member at the National University of Colombia who conducted a workshop on Sept 19-24, 2016 in Tumaco, Columbia. During the workshop, which involved 120 high school girls and 80 minority students, including Afro-Colombian and older students, the students built the wooden microscopes and examined specimens, and compared the microscopes to a conventional light microscope. Assembling the wooden microscopes was found to be a useful procedure that was similar to a scientific protocol, and encouraged young girls and older students to participate in science. This was especially promising in Colombia, where there are few women in science and little effort to increase women in STEM fields.
Another area of outreach emerged recently when one of us, USP, an undergraduate student at Duke University, who was taught by SAE how to assemble the wooden microscopes and how to use the lesson plan, took three wooden microscopes on a visit to her family in Bangalore, India in summer 2018 [2]. There she organized and led three sessions in state run, under-resourced government schools, involving classes of ~25-40 students each. This was very successful – the students enjoyed learning about the microscopes and building them, and the science teachers were interested in expanding the sessions to other government schools. USP taught the teachers how to assemble and use the microscopes and gave the teachers the microscopes and lesson plan, which is also available to the public at the BPS web site. She also met with a founder of the organization, Whitefield Rising, which is working to improve teaching in government schools, and taught her and several volunteers how to assemble the microscopes and conduct the sessions. The Whitefield Rising members have been able to conduct nine further sessions in Bangalore over the past ~18 months (Fig. 3), using microscope kits provided to them by the BPS. USP has continued to work with members of the Whitefield Rising group during her summer and winter breaks on visits to Bangalore. Recently she has been working with another volunteer group that has expanded the outreach efforts to New Delhi.
The light microscopy outreach that our laboratory is conducting in India in collaboration with the BPS is having a positive impact because we have been able to develop a partnership with volunteers in Bangalore and New Delhi. The overall goal is to enhance science education globally, especially in less advantaged schools, by providing a low-cost microscope that can be used to introduce students to scientific concepts.
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Development of a Freeze-Dried CRISPR-Cas12 Sensor for Detecting Wolbachia in the Secondary Science Classroom
Training the future synthetic biology workforce requires the opportunity for students to be exposed to biotechnology concepts and activities in secondary education. Detecting Wolbachia bacteria in arthropods using polymerase chain reaction (PCR) has become a common way for secondary students to investigate and apply recombinant DNA technology in the science classroom. Despite this important activity, cutting-edge biotechnologies such as clustered regularly interspaced short palindromic repeat (CRISPR)-based diagnostics have yet to be widely implemented in the classroom. To address this gap, we present a freeze-dried CRISPR-Cas12 sensing reaction to complement traditional recombinant DNA technology education and teach synthetic biology concepts. The reactions accurately detect Wolbachia from arthropod-derived PCR samples in under 2 h and can be stored at room temperature for over a month without appreciable degradation. The reactions are easy-to-use and cost less than $40 to implement for a classroom of 22 students including the cost of reusable equipment. We see these freeze-dried CRISPR-Cas12 reactions as an accessible way to incorporate synthetic biology education into the existing biology curriculum, which will expand biology educational opportunities in science, technology, engineering, and mathematics.
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- Award ID(s):
- 1936789
- NSF-PAR ID:
- 10326921
- Date Published:
- Journal Name:
- ACS synthetic biology
- Volume:
- 11
- Issue:
- 2
- ISSN:
- 2161-5063
- Page Range / eLocation ID:
- 835–842
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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- Free Publicly Accessible Full Text
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Accepted Manuscript1.0
- Journal Article:
- https://doi.org/https://doi.org/10.1021/acssynbio.1c00503
