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Aneuploidy, or an incorrect chromosome number, is ubiquitous among cancers. Whole-genome duplication, resulting in tetraploidy, often occurs during the evolution of aneuploid tumors. Cancers that evolve through a tetraploid intermediate tend to be highly aneuploid and are associated with poor patient prognosis. The identification and enrichment of tetraploid cells from mixed populations is necessary to understand the role these cells play in cancer progression. Dielectrophoresis (DEP), a label-free electrokinetic technique, can distinguish cells based on their intracellular properties when stimulated above 10 MHz, but DEP has not been shown to distinguish tetraploid and/or aneuploid cancer cells from mixed tumor cell populations. Here, we used high-frequency DEP to distinguish cell subpopulations that differ in ploidy and nuclear size under flow conditions. We used impedance analysis to quantify the level of voltage decay at high frequencies and its impact on the DEP force acting on the cell. High-frequency DEP distinguished diploid cells from tetraploid clones due to their size and intracellular composition at frequencies above 40 MHz. Our findings demonstrate that high-frequency DEP can be a useful tool for identifying and distinguishing subpopulations with nuclear differences to determine their roles in disease progression.
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Benchmarking Automated Rayleigh-Wave Arrival Angle Measurements for USArray Seismograms
Abstract Surface-wave arrival angles are an important secondary set of observables to constrain Earth’s 3D structure. These data have also been used to refine information on the alignments of horizontal seismometer components with the geographic coordinate system. In the past, particle motion has been inspected and analyzed on single three-component seismograms, one at a time. But the advent of large, dense seismic networks has made this approach tedious and impractical. Automated toolboxes are now routinely used for datasets in which station operators cannot determine the orientation of a seismometer upon deployment, such as conventional free-fall ocean bottom seismometers. In a previous paper, we demonstrated that our automated Python-based toolbox Doran–Laske-Orientation-Python compares favorably with traditional approaches to determine instrument orientations. But an open question has been whether the technique also provides individual high-quality measurements for an internally consistent dataset to be used for structural imaging. For this feasibility study, we compared long-period Rayleigh-wave arrival angles at frequencies between 10 and 25 mHz for 10 earthquakes during the first half of 2009 that were recorded at the USArray Transportable Array—a component of the EarthScope program. After vigorous data vetting, we obtained a high-quality dataset that compares favorably with an arrival angle database compiled using our traditional interactive screen approach, particularly at frequencies 20 mHz and above. On the other hand, the presence of strong Love waves may hamper the automated measurement process as currently implemented.
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- Award ID(s):
- 1722579
- PAR ID:
- 10332413
- Date Published:
- Journal Name:
- Seismological Research Letters
- Volume:
- 93
- Issue:
- 2A
- ISSN:
- 0895-0695
- Page Range / eLocation ID:
- 763 to 776
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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- Free Publicly Accessible Full Text
-
Accepted Manuscript1.0
- Journal Article:
- https://doi.org/10.1785/0220210189
