skip to main content
US FlagAn official website of the United States government
dot gov icon
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
https lock icon
Secure .gov websites use HTTPS
A lock ( lock ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Attention:

The DOI auto-population feature in the Public Access Repository (PAR) will be unavailable from 4:00 PM ET on Tuesday, July 8 until 4:00 PM ET on Wednesday, July 9 due to scheduled maintenance. We apologize for the inconvenience caused.


Title: A CURE for Physiological Characterization of Bacterioplankton in Liquid Culture
ABSTRACT Bacterial characterization is an important aspect of microbiology that includes experimentally determining growth rates, environmental conditions conducive to growth, and the types of energy sources microorganisms can use. Researchers use this information to help understand and predict an organism’s ecological distribution and environmental functions. Microbiology students generally conduct bacterial characterization experiments in their coursework; however, they are frequently restricted to model organisms without ecological relevance and already well-studied physiologies. We present a course-based undergraduate research experience (CURE) curriculum to involve students in characterization of previously untested, ecologically relevant aquatic free-living bacteria (bacterioplankton) cultures to identify the usable nutrient substrates, as well as the temperature and salinity ranges conducive to growth. Students use these results to connect their organism’s physiology to the isolation environment. This curriculum also exposes students to advanced microbiology methods such as flow cytometry for measuring cell concentrations, teaches them to use the programming language R for data plotting, and emphasizes scientific communication through writing, speaking, poster creation/presentation, and social media. This CURE is an attractive introduction to scientific research and was successfully tested with 187 students in three semesters at two different universities. Students generated reproducible growth data for multiple strains across these different deployments, demonstrating the utility of the curriculum for research support.  more » « less
Award ID(s):
1945279 1931113
PAR ID:
10334330
Author(s) / Creator(s):
; ; ; ; ; ; ;
Editor(s):
Marvasi, Massimiliano
Date Published:
Journal Name:
Journal of Microbiology & Biology Education
ISSN:
1935-7877
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
More Like this
  1. null (Ed.)
    Course-based undergraduate research experiences (CUREs) often involve a component where the outcomes of student research are broadly relevant to outside stakeholders. We wanted to see if building courses around an environmental justice issue relevant to the local community would impact students’ sense of civic engagement and appreciation of the relevance of scientific research to the community. In this quasi-experimental study, we assessed civic engagement and scientific identity gains ( N = 98) using pre- and post-semester surveys and open-ended interview responses in three different CUREs taught simultaneously at three different universities. All three CURES were focused on an environmental heavy metal pollution issue predominantly affecting African–Americans in Birmingham, Alabama. While we found increases in students’ sense of science efficacy and identity, our team was unable to detect meaningful changes in civic engagement levels, all of which were initially quite high. However, interviews suggested that students were motivated to do well in their research because the project was of interest to outside stakeholders. Our observations suggest that rather than directly influencing students’ civic engagement, the “broadly relevant” component of our CUREs engaged their pre-existing high levels of engagement to increase their engagement with the material, possibly influencing gains in science efficacy and science identity. Our observations are consistent with broader community relevance being an important component of CURE success, but do not support our initial hypothesis that CURE participation would influence students’ attitudes toward the civic importance of science. 
    more » « less
  2. ABSTRACT We previously developed and assessed “The Art of Microbiology,” a course-based undergraduate research experience (CURE) which uses agar art to spur student experimentation, where we found student outcomes related to science persistence. However, these outcomes were not correlated with specific activities and gains were not reported from more than one class. In this study, we explored which of the three major activities in this CURE—agar art, experimental design, or poster presentations—affected student engagement and outcomes associated with improved understanding of the nature of science (NOS). The Art of Microbiology was studied in three microbiology teaching laboratories: at a research university with either the CURE developer (18 students) or a CURE implementer (39 students) and at a community college with a CURE implementer (25 students). Our quasi-experimental mixed methods study used pre/post-NOS surveys and semi-structured class-wide interviews. Community college students had lower baseline NOS responses but had gains in NOS similar to research university students post-CURE. We surveyed research university students following each major activity using the Assessing Student Perspective of Engagement in Class Tool (ASPECT) survey but did not find a correlation between NOS and activity engagement. Of the three activities, we found the highest engagement with agar art, especially in the CURE developer class. Interviewed students in all classes described agar art as a fun, relevant, and low-stakes assignment. This work contributes to the evidence supporting agar art as a curricular tool, especially in ways that can add research to classrooms in and beyond the research university. 
    more » « less
  3. Abstract Course‐based undergraduate research experiences (CUREs) provide students with valuable opportunities to engage in research in a classroom setting, expanding access to research opportunities for undergraduates, fostering inclusive research and learning environments, and bridging the gap between the research and education communities. While scientific practices, integral to the scientific discovery process, have been widely implemented in CUREs, there have been relatively few reports emphasizing the incorporation of core biology concepts into CURE curricula. In this study, we present a CURE that integrates core biology concepts, including genetic information flow, phenotype–genotype relationships, mutations and mutants, and structure–function relationships, within the context of mutant screening and gene loci identification. The design of this laboratory course aligns with key CURE criteria, as demonstrated by data collected through the laboratory course assessment survey (LCAS). The survey of undergraduate research experiences (SURE) demonstrates students' learning gains in both course‐directed skills and transferrable skills following their participation in the CURE. Additionally, concept survey data reflect students' self‐perceived understanding of the aforementioned core biological concepts. Given that genetic mutant screens are central to the study of gene function in biology, we anticipate that this CURE holds potential value for educators and researchers who are interested in designing and implementing a mutant screen CURE in their classrooms. This can be accomplished through independent research or by establishing partnerships between different units or institutions. 
    more » « less
  4. Teaching students how to think like scientists is a critical but challenging goal in biochemistry education. The Biochemistry Authentic Scientific Inquiry Lab (BASIL) initiative was conceived by Dr Paul Craig from the Rochester Institute of Technology and is led by colleagues across multiple institutions. They have developed an innovative curriculum that transforms traditional cookbook-style laboratory courses into authentic research experiences, also known as a Course-based Undergraduate Research Experience (CURE). By investigating real proteins with unknown functions, students learn essential scientific skills while expanding our knowledge of protein biochemistry. 
    more » « less
  5. It is well known that learning occurs best when students are engaged with a topic that interests them or has relevance for important aspects of their lives. In coastal California, the health of the ocean is a serious local concern, and ocean plastics are ubiquitous. We have developed a course‐based undergraduate research experience (CURE) on an existing research project addressing microbes colonizing floating plastic marine debris. The objective of the project is to increase student engagement and persistence in biology. The project (recently awarded an NSF education grant focused on Hispanic students) brings together National University (NU), an undergraduate teaching institution serving non‐traditional students, with Scripps Institution of Oceanography (SIO), a world‐renowned research‐oriented institution at UC San Diego. A modular design allows students from different biology courses (both non‐majors and majors) to participate in field and laboratory research while also interacting with research scientists and graduate students. Module contents range from classroom material including experimental design, hypothesis testing, and data analysis, to laboratory activities such as deployment of test materials, microbiology and molecular biology techniques, as well as bioinformatics. Assessment of the project involves surveys and focus groups to evaluate student engagement, as well as institutional metrics such as retention in the BS Biology program. A pilot involving a non majors general biology course visiting SIO was well‐received by students. Currently (November 2018) an extended intervention is underway with a majors general biology course. During the first week of class students learned about the research project via video material and class lectures. A half day visit to SIO provided them with field trip experience, laboratory activities, presentation about plastic research, and interactions with scientists and graduate students. In successive laboratory activities, students observed colony morphology, performed Gram stainings and colony PCR, practiced Blast searches and developed simple phylogenetic trees. We conclude that the framework can be successfully implemented in spite of time and logistical challenges. We anticipate implementing and disseminating this CURE as a widely applicable model for biology and ocean science education centered on contemporary topics of immediate interest to students. Support or Funding Information This project is funded by the NSF‐HSI grant #1832545 
    more » « less