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Title: Retention and Fouling during Nanoparticle Filtration: Implications for Membrane Purification of Biotherapeutics
One major challenge in the development of nanoparticle-based therapeutics, including viral vectors for the delivery of gene therapies, is the development of cost-effective purification technologies. The objective of this study was to examine fouling and retention behaviors during the filtration of model nanoparticles through membranes of different pore sizes and the effect of solution conditions. Data were obtained with 30 nm fluorescently labeled polystyrene latex nanoparticles using both cellulosic and polyethersulfone membranes at a constant filtrate flux, and both pressure and nanoparticle transmission were evaluated as a function of cumulative filtrate volume. The addition of NaCl caused a delay in nanoparticle transmission and an increase in fouling. Nanoparticle transmission was also a function of particle hydrophobicity. These results provide important insights into the factors controlling transmission and fouling during nanoparticle filtration as well as a framework for the development of membrane processes for the purification of nanoparticle-based therapeutics.  more » « less
Award ID(s):
1822101 1841474
PAR ID:
10335618
Author(s) / Creator(s):
; ; ;
Date Published:
Journal Name:
Membranes
Volume:
12
Issue:
3
ISSN:
2077-0375
Page Range / eLocation ID:
299
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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