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Title: Spatially resolved measurements of ballistic and total transmission in microscale tissue samples from 450 nm to 1624 nm

We built a simple and versatile setup to measure tissue ballistic and total transmission with customizable wavelength range, spatial resolution, and sample sizes. We performed ballistic transmission and total transmission measurements of overlying structures from biological samplesex vivo. We obtained spatially resolved transmission maps to reveal transmission heterogeneity from five microscale tissue samples:Danionellaskin, mouse skull bone, mosquito cuticle, wasp cuticle, and rat dura over a wide spectral range from 450 nm to 1624 nm at a spatial resolution of ∼25µm for ballistic transmission measurements and ∼50µm for total transmission measurements. We expect our method can be straightforwardly applied to measuring the transmission of other samples. The measurement results will be valuable for multiphoton microscopy. The total transmission of a sample is important for the collection of multiphoton excited fluorescence and the assessment of laser-induced sample heating. The ballistic transmission determines the excitation power at the focus and hence the fluorescence signal generation. Therefore, knowledge of ballistic transmission, total transmission, and transmission heterogeneity of overlying structures of animals and organs are essential to determine the optimal excitation wavelength and fluorophores for non-invasive multiphoton microscopy.

 
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Award ID(s):
1719875 1656664
NSF-PAR ID:
10369525
Author(s) / Creator(s):
; ; ; ; ; ;
Publisher / Repository:
Optical Society of America
Date Published:
Journal Name:
Biomedical Optics Express
Volume:
13
Issue:
1
ISSN:
2156-7085
Page Range / eLocation ID:
Article No. 438
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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