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Abstract Much remains to be explored regarding the diversity of uncultured, host-associated microbes. Here, we describe rectangular bacterial structures (RBSs) in the mouths of bottlenose dolphins. DNA staining revealed multiple paired bands within RBSs, suggesting the presence of cells dividing along the longitudinal axis. Cryogenic transmission electron microscopy and tomography showed parallel membrane-bound segments that are likely cells, encapsulated by an S-layer-like periodic surface covering. RBSs displayed unusual pilus-like appendages with bundles of threads splayed at the tips. We present multiple lines of evidence, including genomic DNA sequencing of micromanipulated RBSs, 16S rRNA gene sequencing, and fluorescence in situ hybridization, suggesting that RBSs are bacterial and distinct from the generaSimonsiellaandConchiformibius(familyNeisseriaceae), with which they share similar morphology and division patterning. Our findings highlight the diversity of novel microbial forms and lifestyles that await characterization using tools complementary to genomics such as microscopy.
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The phonology of Guébie
Abstract Guébie is an Eastern Kru language spoken by about 7000 people in the Gagnoa prefecture of Côte d’Ivoire. This paper provides an overview of the phonology of Guébie, including the complex tone system with four contrastive pitch heights, multiple types of vowel harmony, reduplication in multiple morphosyntactic contexts, CVCV/CCV alternations, and the phonotactic behaviour of implosives as sonorant‐like rather than obstruent‐like. Comparisons with other Kru and West African languages are made along the way.
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- Award ID(s):
- 1760302
- PAR ID:
- 10371050
- Publisher / Repository:
- Wiley-Blackwell
- Date Published:
- Journal Name:
- Language and Linguistics Compass
- Volume:
- 16
- Issue:
- 8
- ISSN:
- 1749-818X
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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