An official website of the United States government
Abstract A pulsar’s scintillation bandwidth is inversely proportional to the scattering delay, making accurate measurements of scintillation bandwidth critical to characterize unmitigated delays in efforts to measure low-frequency gravitational waves with pulsar timing arrays. In this pilot work, we searched for a subset of known pulsars within ∼97% of the data taken with the Puerto Rico Ultimate Pulsar Processing Instrument for the AO327 survey with the Arecibo telescope, attempting to measure the scintillation bandwidths in the data set by fitting to the 2D autocorrelation function of their dynamic spectra. We successfully measured 38 bandwidths from 23 pulsars (six without prior literature values), finding that: almost all of the measurements are larger than the predictions from NE2001 and YMW16 (two popular galactic models); NE2001 is more consistent with our measurements than YMW16; Gaussian fits to the bandwidth are more consistent with both electron density models than Lorentzian ones; and for the 17 pulsars with prior literature values, the measurements between various sources often vary by a few factors. The success of Gaussian fits may be due to the use of Gaussian fits to train models in previous work. The variance of literature values over time could relate to the scaling factor used to compare measurements, but also seems consistent with time-varying interstellar medium parameters. This work can be extended to the rest of AO327 to further investigate these trends, highlighting the continuing importance of large archival data sets for projects beyond their initial conception.
more »
« less
Metagenomes and Metagenome-Assembled Genomes from Substrate-Amended Hot Spring Sediment Incubations from Yellowstone National Park
ABSTRACT Here, we report on eight sediment metagenomes obtained from an alkaline hot spring, with their corresponding metagenome-assembled genomes. Samples had been incubated for 48 h with various substrate amendments in conjunction with the amino acid analog l -homopropargylglycine in a study targeted at identifying anabolicly active uncultured thermophilic archaea and bacteria.
more »
« less
- Award ID(s):
- 1736255
- PAR ID:
- 10382134
- Editor(s):
- Newton, Irene L.
- Date Published:
- Journal Name:
- Microbiology Resource Announcements
- Volume:
- 11
- Issue:
- 4
- ISSN:
- 2576-098X
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
More Like this
-
-
null (Ed.)In nature, concentrations of dissolved inorganic carbon (DIC; = CO 2 + HCO 3 - + CO 3 2- ) can be low, and autotrophic organisms adapt with a variety of mechanisms to elevate intracellular DIC concentrations to enhance CO 2 fixation. Such mechanisms have been well-studied in Cyanobacteria , but much remains to be learned about their activity in other phyla. Novel multi-subunit membrane-spanning complexes capable of elevating intracellular DIC were recently described in three species of bacteria. Homologs of these complexes are distributed among 17 phyla in Bacteria and Archaea, and are predicted to consist of one, two, or three subunits. To determine whether DIC accumulation is a shared feature of these diverse complexes, seven of them, representative of organisms from four phyla, from a variety of habitats, and with three different subunit configurations were chosen for study. A high-CO 2 requiring, carbonic anhydrase-deficient ( yadF - cynT - ) strain of E. coli Lemo21(DE3), which could be rescued via elevated intracellular DIC concentrations, was created for heterologous expression and characterization of the complexes. Expression of all seven complexes rescued the ability of E. coli Lemo21(DE3) yadF - cynT - to grow under low CO 2 conditions, and six of the seven generated measurably elevated intracellular DIC concentrations when their expression was induced. For complexes consisting of two or three subunits, all subunits were necessary for DIC accumulation. Isotopic disequilibrium experiments clarified that CO 2 was the substrate for these complexes. In addition, the presence of an ionophore prevented the accumulation of intracellular DIC, suggesting that these complexes may couple proton potential to DIC accumulation. IMPORTANCE To facilitate the synthesis of biomass from CO 2 , autotrophic organisms use a variety of mechanisms to increase intracellular DIC concentrations. A novel type of multi-subunit complex has recently been described, which has been shown to generate measurably elevated intracellular DIC concentrations in three species of bacteria, begging the question of whether these complexes share this capability across the 17 phyla of Bacteria and Archaea where they are found. This study shows that DIC accumulation is a trait shared by complexes with varied subunit structures, from organisms with diverse physiologies and taxonomies, suggesting that this trait is universal among them. Successful expression in E. coli suggests the possibility of their expression in engineered organisms synthesizing compounds of industrial importance from CO 2 .more » « less
-
Abstract Correctly calculating the timing and amount of crop irrigation is crucial for capturing irrigation effects on surface water and energy budgets and land‐atmosphere interactions. This study incorporated a dynamic irrigation scheme into the Noah with multiparameterization land surface model and investigated three methods of determining crop growing season length by agriculture management data. The irrigation scheme was assessed at field scales using observations from two contrasting (irrigated and rainfed) AmeriFlux sites near Mead, Nebraska. Results show that crop‐specific growing‐season length helped capture the first application timing and total irrigation amount, especially for soybeans. With a calibrated soil‐moisture triggering threshold (IRR_CRI), using planting and harvesting dates alone could reasonably predict the first application for maize. For soybeans, additional constraints on growing season were required to correct an early bias in the first modeled application. Realistic leaf area index input was essential for identifying the leaf area index‐based growing season. When transitioning from field to regional scales, the county‐level calibrated IRR_CRI helped mitigate overestimated (underestimated) total irrigation amount in southeastern Nebraska (lower Mississippi River Basin). In these two heavily irrigated regions, irrigation produced a cooling effect of 0.8–1.4 K, a moistening effect of 1.2–2.4 g/kg, a reduction in sensible heat flux by 60–105 W/m2, and an increase in latent heat flux by 75–120 W/m2. Most of irrigation water was used to increase soil moisture and evaporation, rather than runoff. Lacking regional‐scale irrigation timing and crop‐specific parameters makes transferring the evaluation and parameter‐constraint methods from field to regional scales difficult.more » « less
-
processes used in designing and relate them to the metrics used in psychology for idea creativity, ie, novelty and fluency. Our goal was to test the reliability of psychometric measures of creativity to assess creativity in team design. We studied 19 teams of 3 professional engineers that engaged in a one hour-long design task. Design tasks have a greater ecological validity than single repetitive tasks like the AUT and the RAT. Engaging in a design task involves a wide range of cognitive activities, which contribute to creative ideation and to expanding the design space. This study focused on the relationship between the teams’ design idea creativity and design behaviors during the task. We explored to what extent design collaboration between teammates, design evaluation and the co-evolution of the problem-solution space relate to the psychometric measures of idea creativity. Results suggest no specific trend in the correlation between collaboration and idea creativity as measured by the metrics used in psychology, while more cognitive focus on problemsolution co-evolution negatively correlates with these measures of idea creativity. The paper concludes with potential explanations for this lack of correlation.more » « less
-
One powerful theme in complexity theory and pseudorandomness in the past few decades has been the use of lower bounds to give pseudorandom generators (PRGs). However, the general results using this hardness vs. randomness paradigm suffer from a quantitative loss in parameters, and hence do not give nontrivial implications for models where we don’t know super-polynomial lower bounds but do know lower bounds of a fixed polynomial. We show that when such lower bounds are proved using random restrictions, we can construct PRGs which are essentially best possible without in turn improving the lower bounds. More specifically, say that a circuit family has shrinkage exponent Γ if a random restriction leaving a p fraction of variables unset shrinks the size of any circuit in the family by a factor of p Γ + o (1) . Our PRG uses a seed of length s 1/(Γ + 1) + o (1) to fool circuits in the family of size s . By using this generic construction, we get PRGs with polynomially small error for the following classes of circuits of size s and with the following seed lengths: (1) For de Morgan formulas, seed length s 1/3+ o (1) ; (2) For formulas over an arbitrary basis, seed length s 1/2+ o (1) ; (3) For read-once de Morgan formulas, seed length s .234... ; (4) For branching programs of size s , seed length s 1/2+ o (1) . The previous best PRGs known for these classes used seeds of length bigger than n /2 to output n bits, and worked only for size s = O ( n ) [8].more » « less
- Free Publicly Accessible Full Text
-
Accepted Manuscript1.0
- Journal Article:
- https://doi.org/10.1128/mra.01065-21
