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Title: Dissecting Flux Balances to Measure Energetic Costs in Cell Biology: Techniques and Challenges
Life is a nonequilibrium phenomenon: Metabolism provides a continuous supply of energy that drives nearly all cellular processes. However, very little is known about how much energy different cellular processes use, i.e., their energetic costs. The most direct experimental measurements of these costs involve modulating the activity of cellular processes and determining the resulting changes in energetic fluxes. In this review, we present a flux balance framework to aid in the design and interpretation of such experiments and discuss the challenges associated with measuring the relevant metabolic fluxes. We then describe selected techniques that enable measurement of these fluxes. Finally, we review prior experimental and theoretical work that has employed techniques from biochemistry and nonequilibrium physics to determine the energetic costs of cellular processes. Expected final online publication date for the Annual Review of Condensed Matter Physics, Volume 14 is March 2023. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.  more » « less
Award ID(s):
2052305
PAR ID:
10388799
Author(s) / Creator(s):
; ; ;
Date Published:
Journal Name:
Annual Review of Condensed Matter Physics
Volume:
14
Issue:
1
ISSN:
1947-5454
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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