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1. Defining biological stress and stress responses based on principles of physics

   https://doi.org/10.1002/jez.2340  

   Kültz, Dietmar (January 2020, Journal of Experimental Zoology Part A: Ecological and Integrative Physiology)

   Abstract Stress represents a multi‐faceted force that is central for the evolution of life. Organisms evolve while adapting to stress and stressful contexts often represent selective bottlenecks. To understand stress effects on biological systems and corresponding coping strategies it is imperative to properly define stress and the resulting strain that triggers compensatory responses in cells and organisms. Here I am deriving such definitions for biological systems based on principles that are established in physics. The relationship between homeostasis of critical biological variables, the elastic limit, the cellular stress response (CSR), cellular homeostasis response (CHR), system dysregulation, and the breaking point (death of the system) is outlined. Dysregulation of homeostatic set‐points of biological variables perturbs the functional properties of the system, shifting them out of the evolutionarily optimized range. Such shifts are accompanied by elevated rates of macromolecular damage, which represents a nonspecific signal for induction of a universal response, the CSR. The CSR complements the CHR in re‐establishing homeostasis of the system as a whole. Moreover, the CSR is essential for coping with suboptimal conditions while the system is in a dysregulated state and for removing excessive damage that accumulates during such periods. The extreme complexity of biological systems and their emergent properties often necessitate monitoring stress effects on many biological variables simultaneously to properly deduce stress effects on the system as a whole. Therefore, increased utilization of systems biology (omics) approaches for characterizing cellular and organismal stress responses facilitates the reductionist dissection of biological stress response mechanisms. 

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2. Are the ecosystem-level evaporative stress indices representative of evaporative stress of vegetation?

   https://doi.org/10.1016/j.agrformet.2024.110195  

   Raghav, Pushpendra; Kumar, Mukesh (October 2024, Agricultural and Forest Meteorology)

   Evaporative Stress Index (ESI), also sometimes referred as Evaporative Stress Ratio (ESR), has been widely used as an indicator of vegetation evaporative stress, and is often used to track forest and agriculture droughts. Lower the stress, higher is the value of ESI or ESR. The goal of this study is to assess the suitability of these indices for tracking vegetation evaporative stress. As the dynamics of water loss from vegetation through transpiration (T) can be different than that of evapotranspiration (ET) from the ecosystem, it is hypothesized that ESI or ESR may not be sufficiently representative of the vegetation evaporative stress. Using eddy covariance flux tower data of 518 site years, distributed across 49-sites and 9 land covers globally, our findings reveal underestimation of vegetation evaporative stress by ESI during periods of high vapor pressure deficit (VPD) and overestimation during dry, low-VPD periods. The results highlight the need to improve representativeness of ESI for monitoring vegetation evaporative stress. Notably, this may entail accurate estimation of ecosystem T in systems lacking in-situ data, a challenge that warrants further attention. 

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3. Association of acute psychosocial stress with oxidative stress: Evidence from serum analysis

   https://doi.org/10.1016/j.redox.2021.102138  

   Kim, Eunkyoung; Zhao, Zhiling; Rzasa, John Robertson; Glassman, Matthew; Bentley, William E.; Chen, Shuo; Kelly, Deanna L.; Payne, Gregory F. (November 2021, Redox Biology)
4. Myosin and Alpha-actinin regulation of stress fiber contractility under tensile stress

   https://doi.org/10.1038/s41598-023-35675-7  

   Ni, Haoran; Ni, Qin; Papoian, Garegin A; Trache, Andreea; Jiang, Yi (December 2023, Scientific Reports)

   Abstract Stress fibers are actomyosin bundles that regulate cellular mechanosensation and force transduction. Interacting with the extracellular matrix through focal adhesion complexes, stress fibers are highly dynamic structures regulated by myosin motors and crosslinking proteins. Under external mechanical stimuli such as tensile forces, the stress fiber remodels its architecture to adapt to external cues, displaying properties of viscoelastic materials. How the structural remodeling of stress fibers is related to the generation of contractile force is not well understood. In this work, we simulate mechanochemical dynamics and force generation of stress fibers using the molecular simulation platform MEDYAN. We model stress fiber as two connecting bipolar bundles attached at the ends to focal adhesion complexes. The simulated stress fibers generate contractile force that is regulated by myosin motors and$$\alpha$$ $\alpha$-actinin crosslinkers. We find that stress fibers enhance contractility by reducing the distance between actin filaments to increase crosslinker binding, and this structural remodeling ability depends on the crosslinker turnover rate. Under tensile pulling force, the stress fiber shows an instantaneous increase of the contractile forces followed by a slow relaxation into a new steady state. While the new steady state contractility after pulling depends only on the overlap between actin bundles, the short-term contractility enhancement is sensitive to the tensile pulling distance. We further show that this mechanical response is also sensitive to the crosslinker turnover rate. Our results provide new insights into the stress fiber mechanics that have significant implications for understanding cellular adaptation to mechanical signaling. 

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5. Stress–stress correlations reveal force chains in gels

   https://doi.org/10.1063/5.0131473  

   Vinutha, H A; Diaz_Ruiz, Fabiola_Doraly; Mao, Xiaoming; Chakraborty, Bulbul; Del_Gado, Emanuela (March 2023, The Journal of Chemical Physics)

   We investigate the spatial correlations of microscopic stresses in soft particulate gels using 2D and 3D numerical simulations. We use a recently developed theoretical framework predicting the analytical form of stress–stress correlations in amorphous assemblies of athermal grains that acquire rigidity under an external load. These correlations exhibit a pinch-point singularity in Fourier space. This leads to long-range correlations and strong anisotropy in real space, which are at the origin of force-chains in granular solids. Our analysis of the model particulate gels at low particle volume fractions demonstrates that stress–stress correlations in these soft materials have characteristics very similar to those in granular solids and can be used to identify force chains. We show that the stress–stress correlations can distinguish floppy from rigid gel networks and that the intensity patterns reflect changes in shear moduli and network topology, due to the emergence of rigid structures during solidification. 

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