More Like this

1. Therapeutic targeting of transcriptional cyclin-dependent kinases

   https://doi.org/10.1080/21541264.2018.1539615  

   Galbraith, Matthew D.; Bender, Heather; Espinosa, Joaquín M. (November 2018, Transcription)
2. Receptor-like cytoplasmic kinases: orchestrating plant cellular communication

   https://doi.org/10.1016/j.tplants.2024.04.006  

   Hailemariam, Sara; Liao, Chao-Jan; Mengiste, Tesfaye (May 2024, Trends in Plant Science)

   The receptor-like kinase (RLK) family of receptors and the associated receptor-like cytoplasmic kinases (RLCKs) have expanded in plants because of selective pressure from environmental stress and evolving pathogens. RLCKs link pathogen perception to activation of coping mechanisms. RLK–RLCK modules regulate hormone synthesis and responses, reactive oxygen species (ROS) production, Ca2+ signaling, activation of mitogen-activated protein kinase (MAPK), and immune gene expression, all of which contribute to immunity. Some RLCKs integrate responses from multiple receptors recognizing distinct ligands. RLKs/RLCKs and nucleotide-binding domain, leucine-rich repeats (NLRs) were found to synergize, demonstrating the intertwined genetic network in plant immunity. Studies in arabidopsis (Arabidopsis thaliana) have provided paradigms about RLCK functions, but a lack of understanding of crop RLCKs undermines their application. In this review, we summarize current understanding of the diverse functions of RLCKs, based on model systems and observations in crop species, and the emerging role of RLCKs in pathogen and abiotic stress response signaling. 

   more » « less
3. IQGAP1 scaffolding links phosphoinositide kinases to cytoskeletal reorganization

   https://doi.org/10.1016/j.bpj.2022.01.018  

   Yerramilli, V. Siddartha; Ross, Alonzo H.; Scarlata, Suzanne; Gericke, Arne (March 2022, Biophysical Journal)
4. Nudge-nudge, WNK-WNK (kinases), say no more?

   https://doi.org/10.1111/nph.15276  

   Cao-Pham, Anh H.; Urano, Daisuke; Ross-Elliott, Timothy J.; Jones, Alan M. (June 2018, New Phytologist)
5. Activity-based ATP analog probes for bacterial histidine kinases

   https://doi.org/10.1016/bs.mie.2022.01.003  

   Lembke, Hannah K.; Carlson, Erin E. (January 2022, Methods in enzymology)

   Histidine kinases (HKs) are sensor proteins found ubiquitously in prokaryotes. They are the first protein in two-component systems (TCSs), signaling pathways that respond to a myriad of environmental stimuli. TCSs are typically comprised of a HK and its cognate response regulator (RR) which often acts as a transcription factor. RRs will bind DNA and ultimately lead to a cellular response. These cellular outputs vary widely, but HKs are particularly interesting as they are tied to antibiotic resistance and virulence pathways in pathogenic bacteria, making them promising drug targets. We anticipate that HK inhibitors could serve as either standalone antibiotics or antivirulence therapies. Additionally, while the cellular response mediated by the HKs is often well-characterized, very little is known about which stimuli trigger the sensor kinase to begin the phosphorylation cascade. Studying HK activity and enrichment of active HKs through activity-based protein profiling will enable these stimuli to be elucidated, filling this fundamental gap in knowledge. Here, we describe methods to evaluate the potency of putative HK inhibitors in addition to methods to calculate kinetic parameters of various activity-based probes designed for the HKs. 

   more » « less