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Title: Spectroscopic Microtomography in the Short-Wave Infrared Wavelength Range
Spectroscopic microtomography provides the ability to perform 4D (3D structural and 1D chemical) imaging of a thick microscopic specimen. Here, we demonstrate spectroscopic microtomography in the short-wave infrared (SWIR) wavelength using digital holographic tomography, which captures both the absorption coefficient and refractive index. A broadband laser in tandem with a tunable optical filter allows us to scan the wavelength from 1100 to 1650 nm. Using the developed system, we measure human hair and sea urchin embryo samples. The resolution estimated with gold nanoparticles is 1.51 μm (transverse) and 1.57 μm (axial) for the field of view of 307 × 246 μm2. The developed technique will enable accurate and efficient analyses of microscopic specimens that have a distinctive absorption or refractive index contrast in the SWIR range.  more » « less
Award ID(s):
1808331
PAR ID:
10433933
Author(s) / Creator(s):
; ; ;
Date Published:
Journal Name:
Sensors
Volume:
23
Issue:
11
ISSN:
1424-8220
Page Range / eLocation ID:
5164
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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