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1. Surface-programmed microbiome assembly in phycosphere to microplastics contamination

   https://doi.org/10.1016/j.watres.2024.122064  

   Fan, Xuan; Kong, Lingyu; Wang, Jingyi; Tan, Yixiao; Xu, Xiangyang; Li, Mengyan; Zhu, Liang (September 2024, Water Research)

   Recalcitrance in microplastics accounts for ubiquitous white pollution. Of special interest are the capabilities of microorganisms to accelerate their degradation sustainably. Compared to the well-studied pure cultures in degrading natural polymers, the algal-bacterial symbiotic system is considered as a promising candidate for microplastics removal, cascading bottom-up impacts on ecosystem-scale processes. This study selected and enriched the algae-associated microbial communities hosted by the indigenous isolation Desmodesmus sp. in wastewater treatment plants with micro-polyvinyl chloride, polyethylene terephthalate, polyethylene, and polystyrene contamination. Results elaborated that multiple settled and specific affiliates were recruited by the uniform algae protagonist from the biosphere under manifold microplastic stress. Alteration of distinct chemical functionalities and deformation of polymers provide direct evidence of degradation in phycosphere under illumination. Microplastic-induced phycosphere-derived DOM created spatial gradients of aromatic protein, fulvic and humic acid-like and tryptophan components to expanded niche-width. Surface thermodynamic analysis was conducted to simulate the reciprocal and reversible interaction on algal-bacterial and phycosphere-microplastic interface, revealing the enhancement of transition to stable and irreversible aggregation for functional microbiota colonization and microplastics capture. Furthermore, pangenomic analysis disclosed the genes related to the chemotaxis and the proposed microplastics biodegradation pathway in enriched algal-bacterial microbiome, orchestrating the evidence for common synthetic polymer particles and ultimately to confirm the effectiveness and potential. The present study emphasizes the necessity for future endeavors aimed at fully leveraging the potential of algal-bacterial mutualistic systems within sustainable bioremediation strategies targeting the eradication of microplastic waste. 

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2. Microplastics in fish relative to point source proximity and trophic level in an urban river.

   Kazmierczak, E; Happel, A; Hoellein, T (March 2026, Freshwater science)

   Microplastics are widespread in the environment, including in the bodies of freshwater fish, with their concentrations influenced by factors such as proximity to point sources, such as wastewater treatment plants (WWTPs), and trophic level. However, few studies have simultaneously assessed the combined effects of these factors on microplastic abundance in urban stream fish. To do so, we measured microplastics and assessed trophic level via N stable isotope (δ15N) content in 6 species of small-bodied fishes (species = Lepomis macrochirus Rafinesque, 1819, Neogobius melanostomus [Pallas, 1814], Fundulus notatus [Rafinesque, 1820], Pimephales notatus [Rafinesque, 1820], Notemigonus crysoleucas [Mitchill, 1814], and Dorosoma cepedianum [Lesueur, 1818]) collected upstream, at, and downstream of a large WWTP in Chicago, Illinois, USA. Additionally, we analyzed stomach contents for 2 of these species (L. macrochirus and N. melanostomus). Four of the six species exhibited δ15N enrichment at and downstream of the WWTP, indicating prolonged residence times at the study sites (i.e., several weeks). Stomach contents of the 2 species measured supported this pattern, showing high chironomid consumption at the WWTP and variable stomach contents elsewhere. For microplastics, 1 species had higher concentrations near the WWTP, but microplastic concentrations did not differ among locations in the other 5 species. We found no evidence of a relationship between δ15N enrichment and microplastic concentration. Overall, the stable isotope and stomach content results suggest a strong relationship of WWTP effluent with fish diet but not with microplastic concentrations in fish. The results suggest that microplastic concentrations in fish is are shaped by interacting, species-specific factors including behavior (i.e., movement and foraging) and physiology (i.e., egestion rates and feeding mechanisms), in addition to proximity to point sources. Our study highlights the complexity of microplastic infiltration into food webs and underscores the need for further research to disentangle the drivers of microplastic accumulation in aquatic organisms. 

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3. Wastewater treatment alters microbial colonization of microplastics

   https://doi.org/10.1371/journal.pone.0244443  

   Kelly, John J.; London, Maxwell G.; McCormick, Amanda R.; Rojas, Miguel; Scott, John W.; Hoellein, Timothy J. (January 2021, PLOS ONE)

   Nerenberg, Robert (Ed.)

   Microplastics are ubiquitous contaminants in aquatic habitats globally, and wastewater treatment plants (WWTPs) are point sources of microplastics. Within aquatic habitats microplastics are colonized by microbial biofilms, which can include pathogenic taxa and taxa associated with plastic breakdown. Microplastics enter WWTPs in sewage and exit in sludge or effluent, but the role that WWTPs play in establishing or modifying microplastic bacterial assemblages is unknown. We analyzed microplastics and associated biofilms in raw sewage, effluent water, and sludge from two WWTPs. Both plants retained >99% of influent microplastics in sludge, and sludge microplastics showed higher bacterial species richness and higher abundance of taxa associated with bioflocculation (e.g. Xanthomonas ) than influent microplastics, suggesting that colonization of microplastics within the WWTP may play a role in retention. Microplastics in WWTP effluent included significantly lower abundances of some potentially pathogenic bacterial taxa (e.g. Campylobacteraceae ) compared to influent microplastics; however, other potentially pathogenic taxa (e.g. Acinetobacter ) remained abundant on effluent microplastics, and several taxa linked to plastic breakdown (e.g. Klebsiella , Pseudomonas , and Sphingomonas ) were significantly more abundant on effluent compared to influent microplastics. These results indicate that diverse bacterial assemblages colonize microplastics within sewage and that WWTPs can play a significant role in modifying the microplastic-associated assemblages, which may affect the fate of microplastics within the WWTPs and the environment. 

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4. Contaminants of Emerging Concern on Microplastics Found in the Chrysaora chesapeakei of the Patuxent River, Chesapeake Bay, MD

   https://doi.org/10.3390/microplastics4020032  

   Smith, Carol A; Drichko, Natalie; Lorenzo, Miranda; Pramanik, Saroj (June 2025, Microplastics)

   Previously, we reported that microplastic volatile organic compounds are present within the Chrysaora chesapeakei of Chesapeake Bay, MD. In this study, we report the presence of contaminants of emerging concern (CECs) on the hydrophobic surface of microplastic (MP) particles extracted from the C. chesapeakei, detected by Raman spectroscopy and identified by Wiley’s KnowItAll Software with IR & Raman Spectral Libraries. C. chesapeakei encounters various microplastics and emerging contaminants as it floats through the depths of the Patuxent River water column. This study identifies subsuming CECs found directly on microplastics from within C. chesapeakei in the wild using Raman spectroscopy. Among the extracted microplastics, some of the emerging contaminants found on the different microplastics were pesticides, pharmaceuticals, minerals, food derivatives, wastewater treatment chemicals, hormones, and recreational drugs. Our results represent the first of such findings in C. chesapeakei, obtained directly from the field, and indicate C. chesapeakei’s relationship with microplastics, with this species serving as a vector of emerging contaminants through the marine food web. This paper further illustrates a relationship between different types of plastics that attract dissimilar types of emerging pollutants in the same surrounding environmental conditions, underscoring the urgent need for further research to fully understand and mitigate the risks that MPs coexist with contaminants. 

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5. Nutrient recovery from treated wastewater by a hybrid electrochemical sequence integrating bipolar membrane electrodialysis and membrane capacitive deionization

   https://doi.org/10.1039/C9EW00981G  

   Gao, Fei; Wang, Li; Wang, Jie; Zhang, Hongwei; Lin, Shihong (February 2020, Environmental Science: Water Research & Technology)

   The growing needs for sustainable nutrient management and pollution control have motivated the development of novel technologies for nutrient recovery from wastewater. However, most of the existing technologies require extensive use of chemicals and intensive consumption of energy to achieve substantial recovery of nutrients. Herein, we present a hybrid electrochemical sequence integrating two relatively novel electrochemical processes, bipolar membrane electrodialysis (BMED) and membrane capacitive deionization (MCDI), for simultaneous removal of phosphorus and nitrogen. Specifically, the BMED process is employed to alkalify the wastewater to facilitate struvite precipitation and the MCDI process is used to further reduce the ammonia concentration in the effluent and concentrate the excess ammonia to a small stream. The electrochemical sequence is demonstrated to remove ∼89% of phosphorus and ∼77% of ammonia, recovering ∼81% of wastewater as a high-quality effluent that can be discharged or reused. This electrochemical treatment train minimizes chemical use and has competitive energy consumption as compared to electrochemical processes for nutrient recovery from wastewater. 

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