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Title: Accelerated Asymmetric Reaction Screening with Optical Assays
Abstract Asymmetric reaction development often involves optimization of several mutually dependent parameters that affect the product yield and enantiomeric excess. Widely available high-throughput experimentation equipment and optical sensing assays can drastically streamline comprehensive optimization efforts and speed up the discovery process at reduced cost, workload, and waste production. A variety of chiroptical assays that utilize fluorescence, UV, and circular dichroism measurements to determine reaction yields and ee values are now available, enabling the screening of numerous small-scale reaction samples in parallel with multi-well plate technology. Many of these optical methods considerably shorten work-up protocols typically required for traditional asymmetric reaction analysis and some can be directly applied to crude mixtures thus eliminating cumbersome separation and purification steps altogether. 1 Introduction 2 Fluorescence Assays 3 UV Sensing Methods 4 Sensing with Circular Dichroism Probes 5 Hybrid Approaches 6 Optical Analysis with Intrinsically CD-Active Reaction Products 7 Conclusion  more » « less
Award ID(s):
1764135
PAR ID:
10442399
Author(s) / Creator(s):
; ; ;
Date Published:
Journal Name:
Synthesis
Volume:
54
Issue:
11
ISSN:
0039-7881
Page Range / eLocation ID:
2527 to 2538
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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