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SUMMARY Single-cell analysis has transformed our understanding of cellular diversity, offering insights into complex biological systems. Yet, manual data processing in single-cell studies poses challenges, including inefficiency, human error, and limited scalability. To address these issues, we propose the automated workflowcellSight, which integrates high-throughput sequencing in a user-friendly platform. By automating tasks like cell type clustering, feature extraction, and data normalization,cellSightreduces researcher workload, promoting focus on data interpretation and hypothesis generation. Its standardized analysis pipelines and quality control metrics enhance reproducibility, enabling collaboration across studies. Moreover,cellSight’s adaptability supports integration with emerging technologies, keeping pace with advancements in single-cell genomics.cellSightaccelerates discoveries in single-cell biology, driving impactful insights and clinical translation. It is available with documentation and tutorials athttps://github.com/omicsEye/cellSight.
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Introducing “ The Integrator ”: A novel technique to monitor environmental flow systems
Abstract We introduce “The Integrator,” a novel technique to quantify transport and reaction metrics commonly used to characterize flow systems. This development consists of two products: (1)The Integratorsampling device and (2) its supporting mathematical framework, which is compatible with semi‐continuous sensor data. The use ofThe Integratordevice simplifies the logistics of sample collection and greatly reduces the number of samples needed, making it ideal to characterize systems that are: (1) difficult to access, (2) large and thus intractable or highly heterogeneous, and (3) highly instrumented otherwise but where a more holistic, mechanistic understanding may be gained by monitoring one or more currently untracked elements. We tested and validatedThe Integratortechnique using experimental data collected from a heart rate monitor (high‐quality, high‐frequency data in response to known excitation events) and solute tracer experiments conducted in two contrasting (fourth and seventh order) rivers. In theSupporting Information, we provide details concerning the design ofThe Integratordevice used in our field case studies and provide insight into potential improvements. Despite our case studies focus on the analysis of conservative and reactive transport of solutes in rivers, the principles behindThe Integratortechnique can be used to monitor water quality in hyporheic zones, aquifers, wetlands, swamps, karsts, oceans, wastewater treatment plants, pipe networks, and air quality. Furthermore, special arrangements ofIntegratordevices can be used to gather data at spatial and temporal resolutions that are currently unattainable due to high transportation and/or personnel costs.
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- PAR ID:
- 10460287
- Publisher / Repository:
- Wiley Blackwell (John Wiley & Sons)
- Date Published:
- Journal Name:
- Limnology and Oceanography: Methods
- Volume:
- 17
- Issue:
- 7
- ISSN:
- 1541-5856
- Page Range / eLocation ID:
- p. 415-427
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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