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1. Statistical Characteristics of High‐Frequency Gravity Waves Observed by an Airglow Imager at Andes Lidar Observatory

   https://doi.org/10.1029/2022EA002256  

   Cao, Bing; Liu, Alan_Z (June 2022, Earth and Space Science)

   Abstract The long‐term statistical characteristics of high‐frequency quasi‐monochromatic gravity waves are presented using multi‐year airglow images observed at Andes Lidar Observatory (ALO, 30.3°S, 70.7°W) in northern Chile. The distribution of primary gravity wave parameters including horizontal wavelength, vertical wavelength, intrinsic wave speed, and intrinsic wave period are obtained and are in the ranges of 20–30 km, 15–25 km, 50–100 m s⁻¹, and 5–10 min, respectively. The duration of persistent gravity wave events captured by the imager approximately follows an exponential distribution with an average duration of 7–9 min. The waves tend to propagate against the local background winds and show evidence of seasonal variations. In austral winter (May–August), the observed wave occurrence frequency is higher, and preferential wave propagation is equator‐ward. In austral summer (November–February), the wave occurrence frequency is lower, and the waves mostly propagate pole‐ward. Critical‐layer filtering plays a moderate role in determining the preferential propagation direction in certain months, especially for waves with a smaller observed phase speed (less than typical background winds). The observed wave occurrence and preferential propagation direction are related to the locations of convection activities nearby and their relative distance to ALO. However, direct wave generations are less likely due to the large distance between the ALO and convective sources. Other mechanisms such as secondary wave generation and possible ducted propagation should be considered. The estimated mean momentum fluxes have typical values of a few m² s⁻². 

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2. Perfluorocarbon nanodroplet size, acoustic vaporization, and inertial cavitation affected by lipid shell composition in vitro

   https://doi.org/10.1121/10.0014934  

   Welch, Phoebe J.; Li, David S.; Forest, Craig R.; Pozzo, Lilo D.; Shi, Chengzhi (October 2022, The Journal of the Acoustical Society of America)

   Perfluorocarbon nanodroplets (PFCnDs) are ultrasound contrast agents that phase-transition from liquid nanodroplets to gas microbubbles when activated by laser irradiation or insonated with an ultrasound pulse. The dynamics of PFCnDs can vary drastically depending on the nanodroplet composition, including the lipid shell properties. In this paper, we investigate the effect of varying the ratio of PEGylated to non-PEGylated phospholipids in the outer shell of PFCnDs on the acoustic nanodroplet vaporization (liquid to gas phase transition) and inertial cavitation (rapid collapse of the vaporized nanodroplets) dynamics in vitro when insonated with focused ultrasound. Nanodroplets with a high concentration of PEGylated lipids had larger diameters and exhibited greater variance in size distribution compared to nanodroplets with lower proportions of PEGylated lipids in the lipid shell. PFCnDs with a lipid shell composed of 50:50 PEGylated to non-PEGylated lipids yielded the highest B-mode image intensity and duration, as well as the greatest pressure difference between acoustic droplet vaporization onset and inertial cavitation onset. We demonstrate that slight changes in lipid shell composition of PFCnDs can significantly impact droplet phase transitioning and inertial cavitation dynamics. These findings can help guide researchers to fabricate PFCnDs with optimized compositions for their specific applications. 

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3. Targeting diffuse midline gliomas: The promise of focused ultrasound-mediated blood-brain barrier opening

   https://doi.org/10.1016/j.jconrel.2023.11.037  

   Martinez, Payton J; Green, Adam L; Borden, Mark A (January 2024, Journal of Controlled Release)

   Diffuse midline gliomas (DMGs), including diffuse intrinsic pontine glioma, have among the highest mortality rates of all childhood cancers, despite recent advancements in cancer therapeutics. This is partly because, unlike some CNS tumors, the blood-brain barrier (BBB) of DMG tumor vessels remains intact. The BBB prevents the permeation of many molecular therapies into the brain parenchyma, where the cancer cells reside. Focused ultrasound (FUS) with microbubbles has recently emerged as an innovative and exciting technology that non-invasively permeabilizes the BBB in a small focal region with millimeter precision. In this review, current treatment methods and biological barriers to treating DMGs are discussed. State-of-the-art FUS-mediated BBB opening is then examined, with a focus on the effects of various ultrasound parameters and the treatment of DMGs. 

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4. Estimation of Anisotropic Material Properties of Soft Tissue by MRI of Ultrasound-Induced Shear Waves

   https://doi.org/10.1115/1.4046127  

   Guertler, Charlotte A.; Okamoto, Ruth J.; Ireland, Jake A.; Pacia, Christopher P.; Garbow, Joel R.; Chen, Hong; Bayly, Philip V. (March 2020, Journal of Biomechanical Engineering)

   Abstract This paper describes a new method for estimating anisotropic mechanical properties of fibrous soft tissue by imaging shear waves induced by focused ultrasound (FUS) and analyzing their direction-dependent speeds. Fibrous materials with a single, dominant fiber direction may exhibit anisotropy in both shear and tensile moduli, reflecting differences in the response of the material when loads are applied in different directions. The speeds of shear waves in such materials depend on the propagation and polarization directions of the waves relative to the dominant fiber direction. In this study, shear waves were induced in muscle tissue (chicken breast) ex vivo by harmonically oscillating the amplitude of an ultrasound beam focused in a cylindrical tissue sample. The orientation of the fiber direction relative to the excitation direction was varied by rotating the sample. Magnetic resonance elastography (MRE) was used to visualize and measure the full 3D displacement field due to the ultrasound-induced shear waves. The phase gradient (PG) of radially propagating “slow” and “fast” shear waves provided local estimates of their respective wave speeds and directions. The equations for the speeds of these waves in an incompressible, transversely isotropic (TI), linear elastic material were fitted to measurements to estimate the shear and tensile moduli of the material. The combination of focused ultrasound and MR imaging allows noninvasive, but comprehensive, characterization of anisotropic soft tissue. 

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5. Dynamic amplitude modulation of microstimulation evokes biomimetic onset and offset transients and reduces depression of evoked calcium responses in sensory cortices

   https://doi.org/10.1016/j.brs.2023.05.013  

   Hughes, Christopher; Kozai, Takashi (May 2023, Brain Stimulation)

   Background: Intracortical microstimulation (ICMS) is an emerging approach to restore sensation to people with neurological injury or disease. Biomimetic microstimulation, or stimulus trains that mimic neural activity in the brain through encoding of onset and offset transients, could improve the utility of ICMS for brain-computer interface (BCI) applications, but how biomimetic microstimulation affects neural activation is not understood. Current “biomimetic” ICMS trains aim to reproduce the strong onset and offset transients evoked in the brain by sensory input through dynamic modulation of stimulus parameters. Stimulus induced depression of neural activity (decreases in evoked intensity over time) is also a potential barrier to clinical implementation of sensory feedback, and dynamic microstimulation may reduce this effect. Objective: We evaluated how bio-inspired ICMS trains with dynamic modulation of amplitude and/or frequency change the calcium response, spatial distribution, and depression of neurons in the somatosensory and visual cortices. Methods: Calcium responses of neurons were measured in Layer 2/3 of visual and somatosensory cortices of anesthetized GCaMP6s mice in response to ICMS trains with fixed amplitude and frequency (Fixed) and three dynamic ICMS trains that increased the stimulation intensity during the onset and offset of stimulation by modulating the amplitude (DynAmp), frequency (DynFreq), or amplitude and frequency (DynBoth). ICMS was provided for either 1-s with 4-s breaks (Short) or for 30-s with 15-s breaks (Long). Results: DynAmp and DynBoth trains evoked distinct onset and offset transients in recruited neural populations, while DynFreq trains evoked population activity similar to Fixed trains. Individual neurons had heterogeneous responses primarily based on how quickly they depressed to ICMS, where neurons farther from the electrode depressed faster and a small subpopulation (1–5%) were modulated by DynFreq trains. Neurons that depressed to Short trains were also more likely to depress to Long trains, but Long trains induced more depression overall due to the increased stimulation length. Increasing the amplitude during the hold phase resulted in an increase in recruitment and intensity which resulted in more depression and reduced offset responses. Dynamic amplitude modulation reduced stimulation induced depression by 14.6 ± 0.3% for Short and 36.1 ± 0.6% for Long trains. Ideal observers were 0.031 ± 0.009 s faster for onset detection and 1.33 ± 0.21 s faster for offset detection with dynamic amplitude encoding. Conclusions: Dynamic amplitude modulation evokes distinct onset and offset transients, reduces depression of neural calcium activity, and decreases total charge injection for sensory feedback in BCIs by lowering recruitment of neurons during long maintained periods of ICMS. In contrast, dynamic frequency modulation evokes distinct onset and offset transients in a small subpopulation of neurons but also reduces depression in recruited neurons by reducing the rate of activation. 

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