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Title: An Open-Source Tool for Non-telecentric Digital Holographic Microscopy Reconstruction
Digital holographic microscopes operating in non-telecentric mode introduce a spherical wavefront that distorts the sample's phase map, requiring its compensation for undistorted phase measurements. We have developed an open-access reconstruction tool that reconstructs non-telecentric holograms  more » « less
Award ID(s):
2042563
PAR ID:
10487554
Author(s) / Creator(s):
; ;
Publisher / Repository:
Optica Publishing Group
Date Published:
Journal Name:
Optica Imaging Congress (3D, COSI, DH, FLatOptics, IS, pcAOP
Page Range / eLocation ID:
HM2E.4
Format(s):
Medium: X
Location:
Boston, Massachusetts
Sponsoring Org:
National Science Foundation
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  1. ; ; (, PLOS ONE)
    Carretero, Luis (Ed.)
    pyDHM is an open-source Python library aimed at Digital Holographic Microscopy (DHM) applications. The pyDHM is a user-friendly library written in the robust programming language of Python that provides a set of numerical processing algorithms for reconstructing amplitude and phase images for a broad range of optical DHM configurations. The pyDHM implements phase-shifting approaches for in-line and slightly off-axis systems and enables phase compensation for telecentric and non-telecentric systems. In addition, pyDHM includes three propagation algorithms for numerical focusing complex amplitude distributions in DHM and digital holography (DH) setups. We have validated the library using numerical and experimental holograms. 
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  2. ; ; (, PLOS ONE)
    Grulkowski, Ireneusz (Ed.)
    Quantitative phase imaging (QPI) via Digital Holographic microscopy (DHM) has been widely applied in material and biological applications. The performance of DHM technologies relies heavily on computational reconstruction methods to provide accurate phase measurements. Among the optical configuration of the imaging system in DHM, imaging systems operating in a non-telecentric regime are the most common ones. Nonetheless, the spherical wavefront introduced by the non-telecentric DHM system must be compensated to provide undistorted phase measurements. The proposed reconstruction approach is based on previous work from Kemper’s group. Here, we have reformulated the problem, reducing the number of required parameters needed for reconstructing phase images to the sensor pixel size and source wavelength. The developed computational algorithm can be divided into six main steps. In the first step, the selection of the +1-diffraction order in the hologram spectrum. The interference angle is obtained from the selected +1 order. Secondly, the curvature of the spherical wavefront distorting the sample’s phase map is estimated by analyzing the size of the selected +1 order in the hologram’s spectrum. The third and fourth steps are the spatial filtering of the +1 order and the compensation of the interference angle. The next step involves the estimation of the center of the spherical wavefront. An optional final optimization step has been included to fine-tune the estimated parameters and provide fully compensated phase images. Because the proper implementation of a framework is critical to achieve successful results, we have explicitly described the steps, including functions and toolboxes, required for reconstructing phase images without distortions. As a result, we have provided open-access codes and a user interface tool with minimum user input to reconstruct holograms recorded in a non-telecentric DHM system. 
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  3. ; ; (, Optica Imaging Congress (3D, COSI, DH, FLatOptics, IS, pcAOP))
    We have developed a joint phase compensation and autofocusing method for telecentric off-axis Digital Holographic Microscopy (DHM), providing in-focus reconstructed phase images without phase distortions. 
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  4. ; (, OSA Imaging and Applied Optics Congress 2021 (3D, COSI, DH, ISA, pcAOP))
    We present a reconstruction algorithm for digital holographic microscopy (DHM) operating in the telecentric regimen. This strategy rests on the minimization of a cost function to estimate both the numerical reference wave and the quantitative phase information. This algorithm paves the way to a universal DHM reconstruction tool. 
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  5. ; (, Applied Optics)
    This works presents a reconstruction algorithm to recover the complex object information for an off-axis digital holographic microscope (DHM) operating in the telecentric regimen. We introduce an automatic and fast method to minimize a cost function that finds the best numerical conjugated reference beam to compensate the filtered object information, eliminating any undesired phase perturbation due to the tilt between the reference and object waves. The novelties of the proposed approach, to the best of our knowledge, are a precise estimation of the interference angle between the object and reference waves, reconstructed phase images without phase perturbations, and reduced processing time. The method has been validated using a manufactured phase target and biological samples. 
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