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Title: Green Synthesis of Gold Nanoparticles Using Upland Cress and Their Biochemical Characterization and Assessment
This research focuses on the plant-mediated green synthesis process to produce gold nanoparticles (Au NPs) using upland cress (Barbarea verna), as various biomolecules within the upland cress act as both reducing and capping agents. The synthesized gold nanoparticles were thoroughly characterized using UV-vis spectroscopy, surface charge (zeta potential) analysis, scanning electron microscopy-energy-dispersive X-ray spectroscopy (SEM-EDX), atomic force microscopy (AFM), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), and X-ray diffraction (XRD). The results indicated the synthesized Au NPs are spherical and well-dispersed with an average diameter ~11 nm and a characteristic absorbance peak at ~529 nm. EDX results showed an 11.13% gold content. Colloidal Au NP stability was confirmed with a zeta potential (ζ) value of −36.8 mV. X-ray diffraction analysis verified the production of crystalline face-centered cubic gold. Moreover, the antimicrobial activity of the Au NPs was evaluated using Gram-negative Escherichiacoli and Gram-positive Bacillus megaterium. Results demonstrated concentration-dependent antimicrobial properties. Lastly, applications of the Au NPs in catalysis and biomedicine were evaluated. The catalytic activity of Au NPs was demonstrated through the conversion of 4-nitrophenol to 4-aminophenol which followed first-order kinetics. Cellular uptake and cytotoxicity were evaluated using both BMSCs (stem) and HeLa (cancer) cells and the results were cell type dependent. The synthesized Au NPs show great potential for various applications such as catalysis, pharmaceutics, and biomedicine.  more » « less
Award ID(s):
2045738
PAR ID:
10494464
Author(s) / Creator(s):
; ; ; ; ; ; ; ; ; ; ; ; ; ;
Corporate Creator(s):
Editor(s):
This article belongs to the Special Issue Synthesis and Applications of Gold Nanoparticles) Rodolphe Antoine
Publisher / Repository:
Nanomaterials
Date Published:
Journal Name:
Nanomaterials
Volume:
12
Issue:
1
ISSN:
2079-4991
Page Range / eLocation ID:
28
Subject(s) / Keyword(s):
green synthesis nanoparticles catalysis cytotoxicity upland cress
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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