skip to main content
US FlagAn official website of the United States government
dot gov icon
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
https lock icon
Secure .gov websites use HTTPS
A lock ( lock ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Explore Research Products in the PAR
It may take a few hours for recently added research products to appear in PAR search results.


Title: Low-coherence snapshot phase-shifting diffraction phase microscope
We proposed a Wollaston-prism-based snapshot phase-shifting diffraction phase microscope (WP-SPDPM) for low-coherence snapshot quantitative phase imaging and videography. Wollaston prism separates two orthogonally linearly polarized beams with high degrees of polarization at a sufficiently small separation angle; one of the beams passing through a pinhole serves as the reference beam. Four phase-shifted interferograms are simultaneously acquired with a polarization camera to accurately retrieve a high spatial resolution phase map. The system is nearly common-path in configuration and can achieve a large slope range and high accuracy. In addition to the ability to resist environmental noise, the WP-SPDPM is suitable for phase measurement using low-coherence light. The accuracy and large measurable slope range of the proposed system is validated and compared experimentally with a commercial profilometer. We believe WP-SPDPM is a powerful tool for the accurate and robust quantitative phase measurement and has a significant potential of the real-time phase imaging.  more » « less
Award ID(s):
2053754
PAR ID:
10495013
Author(s) / Creator(s):
; ; ; ;
Publisher / Repository:
Optics and Lasers in Engineering
Date Published:
Journal Name:
Optics and Lasers in Engineering
Volume:
167
Issue:
C
ISSN:
0143-8166
Page Range / eLocation ID:
107618
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
More Like this
  1. ; (, Optics Letters)
    We propose a novel and simple snapshot phase-shifting diffraction phase microscope with a polarization grating and spatial phase-shifting technology. Polarization grating separates the incident beam into left and right circular polarization beams, one of which is used as the reference beam after passing through a pinhole. Four phase-shifted interferograms can be captured simultaneously from the polarization camera to reconstruct the high spatial resolution phase map. The principle is presented in this Letter, and the performance of the proposed system is demonstrated experimentally. Due to the near-common-path configuration and snapshot feature, the proposed system provides a feasible way for real-time quantitative phase measurement with minimal sensitivity to vibration and thermal disturbance. 
    more » « less
  2. ; ; ; (, Optics Letters)
    We propose an on-axis deflectometric system for the accurate measurement of freeform surfaces with large slope ranges. A miniature plane mirror is attached on the illumination screen to fold the optical path and achieve the on-axis deflectometric testing. Due to the existence of the miniature folding mirror, the deep-learning method is applied to recover the missing surface data in a single measurement. Low sensitivity to the calibration error of system geometry and high testing accuracy can be achieved with the proposed system. The feasibility and accuracy of the proposed system have been validated. The system is low in cost and simple in configuration, and it provides a feasible way for the flexible and general testing of freeform surfaces, with a significant potential of the application in on-machine testing. 
    more » « less
  3. ; (, Polarization Science and Remote Sensing IX)
    Channeled spectropolarimetry measures the spectral dependence of the polarization states of light. This technique is marked by its snapshot feature, in that the complete polarization states can be determined simultaneously from a single intensity spectrum. However, without athermalization, it suffers from high sensitivity to temperature, which in turn, degrades the polarimetric reconstruction accuracy. In this paper, we present a calibration technique for a fiber-based channeled spectropolarimetry that leverages phase-shifting interferometry to accurately demodulate the retarders' phase, thereby improving the accuracy of the acquired Stokes parameters. Additionally, it enables robust spectropolarimetric performance that is insensitive to environmental perturbations. Experimental results demonstrate that calibrations using phase-shifting interferometry improve the Stokes reconstruction results by approximately a factor of 3 when compared to the reference beam calibration method. 
    more » « less
  4. ; ; ; ; ; ; ; ; ; ; et al (, Advanced Optical Materials)
    Abstract Fourier ptychography (FP) is an enabling imaging technique that produces high‐resolution complex‐valued images with extended field coverages. However, when FP images a phase object with any specific spatial frequency, the captured images contain only constant values, rendering the recovery of the corresponding linear phase ramp impossible. This challenge is not unique to FP but also affects other common microscopy techniques — a rather counterintuitive outcome given their widespread use in phase imaging. The underlying issue originates from the non‐uniform phase transfer characteristic inherent in microscope systems, which impedes the conversion of object wavefields into discernible intensity variations. To address this challenge, spatially‐coded Fourier ptychography (scFP) is presented for true quantitative phase imaging. In scFP, a flexible and detachable coded thin film is attached atop the image sensor in a regular FP setup. The spatial modulation of this thin film ensures a uniform phase response across the entire synthetic bandwidth. It improves reconstruction quality, corrects refractive index underestimation issues prevalent in conventional FP, and adds a new dimension of measurement diversity in spatial domain. The development of scFP is expected to catalyze new research directions and applications for phase imaging, emphasizing the need for true quantitative accuracy with uniform frequency response. 
    more » « less
  5. ; ; ; (, Communications Physics)
    Abstract There is a significant gap in cost-effective quantitative phase microscopy (QPM) systems for studying dynamic cellular processes while maintaining accuracy for long-term cellular monitoring. Current QPM systems often rely on complex and expensive voltage-controllable components like Spatial Light Modulators or two-beam interferometry. To address this, we introduce a QPM system optimized for time-varying phase samples using azobenzene liquid crystal as a Zernike filter with a polarization-sensing camera. This system operates without input voltage or moving components, reducing complexity and cost. Optimized for gentle illumination to minimize phototoxicity, it achieves a 1 Hz frame rate for prolonged monitoring. The system demonstrated accuracy with a maximum standard deviation of ±42 nm and low noise fluctuations of ±2.5 nm. Designed for simplicity and single-shot operations, our QPM system is efficient, robust, and precisely calibrated for reliable measurements. Using inexpensive optical components, it offers an economical solution for long-term, noninvasive biological monitoring and research applications. 
    more » « less
  • Citation Formats
  • MLA
  • APA
  • Chicago
  • BibTeX
  • Save / Share this Record
  • Send to Email