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Title: A chemically-defined growth medium to support Lactobacillus-Acetobacter sp. community analysis
Lactobacilli andAcetobacter sp.are commercially important bacteria that often form communities in natural fermentations, including food preparations, spoilage, and in the digestive tract of the fruit fly Drosophila melanogaster. Communities of these bacteria are widespread and prolific, despite numerous strain-specific auxotrophies, suggesting they have evolved nutrient interdependencies that regulate their growth. The use of a chemically-defined medium (CDM) supporting the growth of both groups of bacteria would facilitate the identification of the molecular mechanisms for the metabolic interactions between them. While numerous CDMs have been developed that support specific strains of lactobacilli orAcetobacter, there has not been a medium formulated to support both genera. We developed such a medium, based on a previous CDM designed for growth of lactobacilli, by modifying the nutrient abundances to improve growth yield. We further simplified the medium by substituting casamino acids in place of individual amino acids and the standard Wolfe’s vitamins and mineral stocks in place of individual vitamins and minerals, resulting in a reduction from 40 to 8 stock solutions. These stock solutions can be used to prepare several CDM formulations that support robust growth of numerous lactobacilli andAcetobacters. Here, we provide the composition and several examples of its use, which is important for tractability in dissecting the genetic and metabolic basis of natural bacterial species interactions.  more » « less
Award ID(s):
2032985 2144342
PAR ID:
10496378
Author(s) / Creator(s):
; ; ; ; ; ; ; ; ; ;
Editor(s):
Al Kassaa, Imad
Publisher / Repository:
PLOS One
Date Published:
Journal Name:
PLOS ONE
Volume:
18
Issue:
10
ISSN:
1932-6203
Page Range / eLocation ID:
e0292585
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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