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The Department of Biological Sciences at Minnesota State University, Mankato has recently implemented a first year undergraduate research experience (called the Research Immersive Scholastic Experience in Biology program; RISEbio) designed to improve student success and engagement in biology. In this program, first year students exchange introductory biology labs for the RISEbio curriculum, where they learn basic laboratory, analytical and scientific reasoning skills before beginning authentic mentored research projects in their second and third semesters. Students in one of three research tracks examine the neural control of reproductive behavior by examining gene expression in the brain of the seasonally breeding green anole lizard (Anolis carolinensis). Working in groups, students gain experience with bioinformatics by examining preliminary RNA-seq data and selecting a gene of interest. Then, students design and test primers to amplify their gene of interest, followed by isolating RNA from the hypothalamus of breeding and non-breeding lizard brains. Lastly, students utilize their isolated RNA samples and validated primers in quantitative RT-PCR studies to determine if their gene of interest is differentially expressed in the anole brain. Preliminary work has identified melatonin receptor 1A (MTNR1A) as more highly expressed in the breeding compared to non-breeding anole hypothalamus, while corticotropin releasing hormone binding protein (CRHBP) expression does not differ seasonally. Together with other aspects of the RISEbio program, these early research experiences have led to increased student outcomes, including increased academic success and enhanced scientific motivation.
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A review of the reproductive biology of mormyroid fishes: An emerging model for biomedical research
Abstract Mormyroidea is a superfamily of weakly electric African fishes with great potential as a model in a variety of biomedical research areas including systems neuroscience, muscle cell and craniofacial development, ion channel biophysics, and flagellar/ciliary biology. However, they are currently difficult to breed in the laboratory setting, which is essential for any tractable model organism. As such, there is a need to better understand the reproductive biology of mormyroids to breed them more reliably in the laboratory to effectively use them as a biomedical research model. This review seeks to (1) briefly highlight the biomedically relevant phenotypes of mormyroids and (2) compile information about mormyroid reproduction including sex differences, breeding season, sexual maturity, gonads, gametes, and courtship/spawning behaviors. We also highlight areas of mormyroid reproductive biology that are currently unexplored and/or have the potential for further investigation that may provide insights into more successful mormyroid laboratory breeding methods.
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- Award ID(s):
- 2243230
- PAR ID:
- 10496727
- Publisher / Repository:
- Wiley
- Date Published:
- Journal Name:
- Journal of Experimental Zoology Part B: Molecular and Developmental Evolution
- ISSN:
- 1552-5007
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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- Free Publicly Accessible Full Text
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Accepted Manuscript1.0
- Journal Article:
- https://doi.org/10.1002/jez.b.23242
