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Title: Adaptation of CUT&RUN for use in African trypanosomes
This Cleavage Under Targets and Release Using Nuclease (CUT&RUN) protocol produces genomic occupancy data for a protein of interest in the protozoan parasiteTrypanosoma brucei. The data produced is analyzed in a similar way as that produced by ChIP-seq. While we describe the protocol for parasites carrying an epitope tag for the protein of interest, antibodies against the native protein could be used for the same purpose.  more » « less
Award ID(s):
2041395
PAR ID:
10507576
Author(s) / Creator(s):
; ; ; ;
Editor(s):
Li, Zhiming
Publisher / Repository:
PLoS
Date Published:
Journal Name:
PLOS ONE
Volume:
18
Issue:
11
ISSN:
1932-6203
Page Range / eLocation ID:
e0292784
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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