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The statistical region merging (SRM) method for image segmentation is based on some solid probabilistic and statistical principles. It produces good segmentation results, and is efficient in term of the computational time. The original SRM algorithm is for Cartesian images sampled by square lattices (sqL). Because hexagonal lattices (hexL) have the advantage that each lattice point in a hexL has six equidistant adjacent lattice points, in this paper, we perform image segmentation for hexagonally sampled images using SRM. We first convert the SRM algorithm from sqLs to hexLs. Then we use some test images to compare the corresponding segmentation effect for hexLs versus sqLs. The experimental results have shown that a hexL exhibits evidently better image segmentation effect than the corresponding sqL (with the same spatial sampling rate as the hexL) using the usual 4-connectivity. Finally, we point out that CT image segmentation may benefit from using hexLs since they provide better image reconstruction effect than sqLs.
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Segmentation for Images of a Single Stem Cell Using Morphological Operations and Statistical Region Merging
For some images of a single stem cell, the boundary delineating the cell is discontinuous or blurry at some locations. If the statistical region merging (SRM) method is applied directly on such images, the image segmentation results may not be ideal. In this paper, for each such image, we add some gradient information into the image; then apply a discontinuous filter on the image so that the image is smoothed a bit and the edges of the image are kept well. Next, closing operations of morphology are applied on the filtered image; and the processed image is segmented using SRM. Finally, apply a threshold on the segmented image to obtain a binary image; apply a hole-filling function to the binary image; extract the biggest connected component in the hole-filled image; and apply a linear transform on the image of the biggest component to match the input image as well as possible in terms of the least squares fitting. This transformed image is the segmentation result. We have applied SRM using connectivity of 4 and 8 as well as a hexagonal lattice. The corresponding segmentation results are tabulated for convenient comparisons; and the results can show that the proposed method may be helpful for the segmentation of such images.
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- Award ID(s):
- 2000158
- PAR ID:
- 10510055
- Publisher / Repository:
- IEEE
- Date Published:
- ISBN:
- 979-8-3503-9793-2
- Page Range / eLocation ID:
- 171 to 175
- Subject(s) / Keyword(s):
- image segmentation statistical region merging morphological operations hexagonal sampling image filters
- Format(s):
- Medium: X
- Location:
- Wuxi, China
- Sponsoring Org:
- National Science Foundation
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- Free Publicly Accessible Full Text
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Accepted Manuscript1.0
- Conference Paper:
- https://doi.org/10.1109/ICSIP57908.2023.10270995
