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Albumin–ruthenium catalyst conjugate for bio-orthogonal uncaging of alloc group
The employment of antibodies as a targeted drug delivery vehicle has proven successful which is exemplified by the emergence of antibody–drug conjugates (ADCs). However, ADCs are not without their shortcomings. Improvements may be made to the ADC platform by decoupling the cytotoxic drug from the delivery vehicle and conjugating an organometallic catalyst in its place. The resulting protein–metal catalyst conjugate was designed to uncage the masked cytotoxin administered as a separate entity. Macropinocytosis of albumin by cancerous cells suggests the potential of albumin acting as the tumor-targeting delivery vehicle. Herein reported are the first preparation and demonstration of ruthenium catalysts with cyclopentadienyl and quinoline-based ligands conjugated to albumin. The effective uncaging abilities were demonstrated on allyloxy carbamate (alloc)-protected rhodamine 110 and doxorubicin, providing a promising catalytic scaffold for the advancement of selective drug delivery methods in the future.
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- Award ID(s):
- 2102225
- PAR ID:
- 10512771
- Editor(s):
- Silverman, Scott
- Publisher / Repository:
- Royal Society of Chemistry
- Date Published:
- Journal Name:
- Organic & Biomolecular Chemistry
- Volume:
- 22
- Issue:
- 15
- ISSN:
- 1477-0520
- Page Range / eLocation ID:
- 2992 to 3000
- Subject(s) / Keyword(s):
- bioorthogonal, prodrug, artificial metalloenzyme, uncaging
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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- Free Publicly Accessible Full Text
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Accepted Manuscript1.0
- Journal Article:
- https://doi.org/10.1039/D4OB00234B
