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Title: Whole Genome Assembly and Annotation of Blackstripe Livebearer Poeciliopsis prolifica
Abstract The blackstripe livebearer Poeciliopsis prolifica is a live-bearing fish belonging to the family Poeciliidae with high level of postfertilization maternal investment (matrotrophy). This viviparous matrotrophic species has evolved a structure similarly to the mammalian placenta. Placentas have independently evolved multiple times in Poeciliidae from nonplacental ancestors, which provide an opportunity to study the placental evolution. However, there is a lack of high-quality reference genomes for the placental species in Poeciliidae. In this study, we present a 674 Mb assembly of P. prolifica in 504 contigs with excellent continuity (contig N50 7.7 Mb) and completeness (97.2% Benchmarking Universal Single-Copy Orthologs [BUSCO] completeness score, including 92.6% single-copy and 4.6% duplicated BUSCO score). A total of 27,227 protein-coding genes were annotated from the merged datasets based on bioinformatic prediction, RNA sequencing and homology evidence. Phylogenomic analyses revealed that P. prolifica diverged from the guppy (Poecilia reticulata) ∼19 Ma. Our research provides the necessary resources and the genomic toolkit for investigating the genetic underpinning of placentation.  more » « less
Award ID(s):
1754669
PAR ID:
10515549
Author(s) / Creator(s):
; ; ;
Editor(s):
Wheat, Christopher
Publisher / Repository:
Oxford University Press
Date Published:
Journal Name:
Genome Biology and Evolution
Volume:
15
Issue:
11
ISSN:
1759-6653
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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